Wang Wenhui, Wang Li, She Junjun, Zhu Jun
Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Sema4, a Mount Sinai venture, Stamford, CT 06902, USA.
Cancer Biol Med. 2021 Aug 17;19(1):30-42. doi: 10.20892/j.issn.2095-3941.2020.0762.
Tumor tissues contain both tumor and non-tumor cells, which include infiltrated immune cells and stromal cells, collectively called the tumor microenvironment (TME). Single-cell RNA sequencing (scRNAseq) enables the examination of heterogeneity of tumor cells and TME. In this review, we examined scRNAseq datasets for multiple cancer types and evaluated the heterogeneity of major cell type composition in different cancer types. We further showed that endothelial cells and fibroblasts/myofibroblasts in different cancer types can be classified into common subtypes, and the subtype composition is clearly associated with cancer characteristic and therapy response.
肿瘤组织包含肿瘤细胞和非肿瘤细胞，后者包括浸润的免疫细胞和基质细胞，统称为肿瘤微环境（TME）。单细胞RNA测序（scRNAseq）能够检测肿瘤细胞和TME的异质性。在本综述中，我们检查了多种癌症类型的scRNAseq数据集，并评估了不同癌症类型中主要细胞类型组成的异质性。我们进一步表明，不同癌症类型中的内皮细胞和成纤维细胞/肌成纤维细胞可分为常见亚型，且亚型组成与癌症特征和治疗反应明显相关。
