Hydrogen Attenuates Myocardial Injury in Rats by Regulating Oxidative Stress and NLRP3 Inflammasome Mediated Pyroptosis.

Hydrogen (H) is an antioxidant with anti-inflammatory and apoptosis functions.This study aimed to estimate the effects of H on acute myocardial infarction (AMI) in rats and its association with the inhibition of oxidative stress and cardiomyocyte pyroptosis. Sixty-four rats were randomly divided into three groups (Sham, AMI, and H). The left anterior descending coronary artery (LAD) of rats in the AMI and H groups was ligated, while rats in the Sham group were threaded without ligation. In addition, 2% H was administered by inhalation for 24 h after ligation in the H group. Transthoracic echocardiography was performed after H inhalation, followed by collection of the serum and cardiac tissue of all rats. H inhalation ameliorated the cardiac dysfunction, infarct size and inflammatory cell infiltration caused by AMI. Meanwhile, H inhalation reduced the concentration of serum Troponin I (TnI), brain natriuretic peptide (BNP), reactive oxygen species (ROS), cardiac malondialdehyde (MDA), and 8-OHdG. In addition, H inhalation inhibited cardiac inflammation and pyroptosis relative proteins expression. H effectively promoted heart functions in AMI rats by regulating oxidative stress and pyroptosis.