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采用酶联免疫吸附测定技术测定含有干果的产品中的展青霉素 干果中的展青霉素

Determination of patulin in products containing dried fruits by Enzyme-Linked Immunosorbent Assay technique Patulin in dried fruits.

作者信息

Przybylska Anna, Chrustek Agnieszka, Olszewska-Słonina Dorota, Koba Marcin, Kruszewski Stefan

机构信息

Department of Toxicology and Bromatology Faculty of Pharmacy L. Rydygier Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun Bydgoszcz Poland.

Department of Pathobiochemistry and Clinical Chemistry Faculty of Pharmacy L. Rydygier Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun Bydgoszcz Poland.

出版信息

Food Sci Nutr. 2021 Jun 21;9(8):4211-4220. doi: 10.1002/fsn3.2386. eCollection 2021 Aug.

Abstract

The era of globalization causes that the export and import of food from different continents of the world are becoming more and more common, which may directly contribute to the increase in pollution in them. The presence of mycotoxin in food is an ubiquitous problem. There is very limited information on the possible influence of the composition of herbal mixtures on the presence of mycotoxins in them, which is an area where research can be expanded. The aim of this study was to determine patulin (PAT) in commercial products containing dried elderberry, rose, blueberry, rowan, hawthorn, and chokeberry fruits by enzyme-linked immunosorbent assay technique. Research using this technique allowed for considering the possible influence of the composition of herbal mixtures on the concentration of patulin in them. Patulin was detected in all analyzed samples with wide range of <LOD ÷ 4,102.0 µg/kg. In 91% of the single-ingredient products, the mean patulin concentration below 50 µg/kg was found. We observed that patulin content in products containing only rose, elderberry, blueberry, rowan, hawthorn, or chokeberry fruit was lower than in herbal blends. Research showed that adding dried rowan fruits to herbal blends may contribute to a decrease in PAT levels (r = 0.8581;  = .0031). Looking for such technological methods creating the most unfavorable conditions for the biosynthesis of patulin in medicinal raw materials is extremely important from the point of view of medical and pharmaceutical care.

摘要

全球化时代使得世界不同大洲之间的食品进出口变得越来越普遍,这可能直接导致食品污染增加。食品中霉菌毒素的存在是一个普遍存在的问题。关于草药混合物成分对其中霉菌毒素存在可能产生的影响,相关信息非常有限,这是一个可以拓展研究的领域。本研究的目的是通过酶联免疫吸附测定技术测定含有干接骨木果、玫瑰果、蓝莓、花楸果、山楂果和酸果蔓果的商业产品中的展青霉素(PAT)。使用该技术进行的研究有助于考虑草药混合物成分对其中展青霉素浓度的可能影响。在所有分析样品中均检测到展青霉素,其含量范围很广,为<LOD÷4,102.0μg/kg。在91%的单一成分产品中,展青霉素平均浓度低于50μg/kg。我们观察到,仅含有玫瑰果、接骨木果、蓝莓、花楸果、山楂果或酸果蔓果的产品中展青霉素含量低于草药混合物。研究表明,在草药混合物中添加干花楸果可能有助于降低展青霉素水平(r = 0.8581;P = 0.0031)。从医疗和制药护理的角度来看,寻找这样的技术方法,为药用原料中展青霉素的生物合成创造最不利的条件极为重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04a2/8358369/f03ba4df3198/FSN3-9-4211-g002.jpg

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