将 CD4 T 细胞优化后注入小鼠的侧脑室。
An optimized intracerebroventricular injection of CD4 T cells into mice.
机构信息
The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel.
The National Institute of Biotechnology in the Negev, Zlotowski Neuroscience Center, and Regenerative Medicine and Stem Cell Research Center, Ben-Gurion University of the Negev, Beer-Sheva 8410501, Israel.
出版信息
STAR Protoc. 2021 Aug 6;2(3):100725. doi: 10.1016/j.xpro.2021.100725. eCollection 2021 Sep 17.
The blood-brain barrier acts as a major barrier for the entrance of most therapeutics into the brain, impeding treatment for neurological disorders. Intracerebroventricular (ICV) injection of T cells is a useful tool for cell therapy of neurological disorders including neurodegenerative and neuropsychiatric diseases and brain tumors. Here, we present an optimized ICV injection of T cells with improved injection efficiency at pathological sites within the brain parenchyma. We describe details of the surgical procedure and verification of injection via immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Fisher et al. (2014); Strominger et al., (2018); Mittal et al. (2019); Eremenko et al. (2019).
血脑屏障是大多数治疗药物进入大脑的主要障碍，阻碍了对神经疾病的治疗。脑室内（ICV）注射 T 细胞是治疗神经疾病（包括神经退行性和神经精神疾病以及脑肿瘤）的细胞疗法的有用工具。在这里，我们提出了一种优化的 T 细胞脑室内注射方法，可提高脑实质内病理部位的注射效率。我们描述了手术过程的细节和通过免疫组织化学验证注射的方法。如需了解本方案的详细使用和执行情况，请参考 Fisher 等人（2014 年）、Strominger 等人（2018 年）、Mittal 等人（2019 年）和 Eremenko 等人（2019 年）。
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