Medical School of Chinese PLA.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2021 Jun 25;50(3):290-297. doi: 10.3724/zdxbyxb-2021-0004.
To investigate the effect of multiple propofol anesthesia and operative trauma on neuroinflammation and cognitive function in development rats and its mechanism. A total of 104 13-day-old neonatal Sprague-Dawley rats were randomly divided into 4 groups with 26 rats in each group: control group was treated with saline q.d for propofol group was treated with propofol q.d for surgery group received abdominal surgery under local anesthesia and then treated with saline q.d for surgery with propofol group received propofol anesthesia plus abdominal surgery under local anesthesia with ropivacaine at d1, then treated with propofol q.d for At d2 of experiment, 13 rats from each group were sacrificed and brain tissue samples were taken, the concentration of TNF-α in hippocampus was detected with ELISA, the expression of caspase-3 and c-fos in hippocampal tissue was determined with immunohistochemical method, the number of apoptotic neurons in hippocampus was examined with TUNEL assay. Morris water maze test was used to examine the cognitive function of the rest rats at the age of 60 d, and the TNF-α concentration, caspase-3, c-fos expressions and the number of apoptotic neurons in hippocampus were also detected. Compared with control group, TNF-α concentration, caspase-3, c-fos expression and the neuroapoptosis in hippocampus increased significantly in other three groups (all <0.05). Compared with surgery group, propofol group and surgery with propofol group showed increased TNF-α level, caspase-3 and c-fos expressions and apoptotic cell numbers (all <0.05), but there was no significant difference between last two groups (all >0.05). Morris water maze test showed that there were no significant differences in swimming speed, escape latency, target quadrant residence time and crossing times among groups (all >0.05). TNF-α level, expressions of caspase-3 and c-fos and apoptotic cell numbers in hippocampus had no significant differences among the 4 adult rats groups (all >0.05). Abdominal surgery and multiple propofol treatment can induce neuroinflammation and neuroapoptosis in hippocampus of neonatal rats, however, which may not cause adverse effects on neurodevelopment and cognitive function when they grown up.
为了研究多次异丙酚麻醉和手术创伤对发育期大鼠神经炎症和认知功能的影响及其机制。将 104 只 13 日龄新生 Sprague-Dawley 大鼠随机分为 4 组，每组 26 只：对照组给予生理盐水 q.d 治疗；异丙酚组给予异丙酚 q.d 治疗；手术组在局部麻醉下行腹部手术，然后给予生理盐水 q.d 治疗；手术加异丙酚组在局部麻醉下行腹部手术，然后给予罗哌卡因和异丙酚治疗，于实验第 1 天，然后给予异丙酚 q.d 治疗。每组各取 13 只大鼠处死，取脑组织标本，采用 ELISA 法检测海马组织 TNF-α 浓度，免疫组化法检测海马组织 caspase-3、c-fos 表达，TUNEL 法检测海马区凋亡神经元数。于 60d 时，用 Morris 水迷宫检测其余大鼠的认知功能，并检测海马 TNF-α 浓度、caspase-3、c-fos 表达及海马区凋亡神经元数。与对照组比较，其余 3 组 TNF-α 浓度、caspase-3、c-fos 表达及海马区神经细胞凋亡均明显增加（均<0.05）；与手术组比较，异丙酚组和手术加异丙酚组 TNF-α 水平、caspase-3 和 c-fos 表达及凋亡细胞数均明显增加（均<0.05），但后 2 组比较差异无统计学意义（均>0.05）。Morris 水迷宫试验结果显示，各组大鼠游泳速度、逃避潜伏期、目标象限停留时间和穿越平台次数比较差异均无统计学意义（均>0.05）。4 组成年大鼠海马 TNF-α 水平、caspase-3 和 c-fos 表达及凋亡细胞数比较差异均无统计学意义（均>0.05）。腹部手术和多次异丙酚处理可诱导新生大鼠海马神经炎症和神经细胞凋亡，但长大后可能不会对神经发育和认知功能产生不良影响。
