Gong Yi-Zhen, Ma Hui, Ruan Guo-Tian, Zhu Li-Chen, Liao Xi-Wen, Wang Shuai, Yan Ling, Huang Wei, Huang Ke-Tuan, Xie Hailun, Zhu Guang-Zhi, Wang Xiang-Kun, Liao Cun, Gao Feng
Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.
Department of Immunology, School of Preclinical Medicine, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, People's Republic of China.
J Cancer. 2021 Jul 25;12(18):5506-5518. doi: 10.7150/jca.51524. eCollection 2021.
The objective was to identify and validate C-X-C motif chemokine ligand 1() for diagnosis and prognosis in colon adenocarcinoma (COAD). Our current study had enrolled one The Cancer Genome Atlas (TCGA) cohort and two Guangxi cohorts to identify and verify the diagnostic and prognostic values of in COAD. Functional enrichment was performed by gene set enrichment analysis (GSEA). In TCGA cohort, the expression of was significantly up-regulated in tumor tissues and decreased as the tumor stage developed. The receiver operating characteristic (ROC) curve showed that had a high diagnostic value for COAD. The result of Kaplan-Meier survival analysis showed that gene expression (=0.045) was significantly correlated with overall survival (OS) of COAD. Results of Guangxi cohort also verified the diagnostic value of in COAD, and sub-group survival analyses also suggested that patients with high expression were related to a favorable OS (Corrected =0.005). GSEA revealed that high expression phenotype was related to cytokine activity, cell apoptosis, regulation pathway, and regulation of autophagy in COAD. In this study, we found that gene might be a potential diagnostic biomarker for COAD, and might serve as a prognostic biomarker for specific subgroup of COAD.
目的是鉴定并验证C-X-C基序趋化因子配体1（）在结肠腺癌（COAD）诊断和预后中的作用。我们目前的研究纳入了一个癌症基因组图谱（TCGA）队列和两个广西队列，以鉴定并验证其在COAD中的诊断和预后价值。通过基因集富集分析（GSEA）进行功能富集。在TCGA队列中，其在肿瘤组织中的表达显著上调，并随着肿瘤分期的发展而降低。受试者工作特征（ROC）曲线显示，其对COAD具有较高的诊断价值。Kaplan-Meier生存分析结果显示，基因表达（=0.045）与COAD的总生存期（OS）显著相关。广西队列的结果也验证了其在COAD中的诊断价值，亚组生存分析还表明，高表达患者的OS较好（校正后=0.005）。GSEA显示，高表达表型与COAD中的细胞因子活性、细胞凋亡、调控途径和自噬调控有关。在本研究中，我们发现基因可能是COAD的潜在诊断生物标志物，并可能作为COAD特定亚组的预后生物标志物。
