人类5S RNA的一个变异基因和一个假基因在体外具有转录活性。
A variant gene and a pseudogene for human 5S RNA are transcriptionally active in vitro.
作者信息
Arnold G J, Kahnt B, Herrenknecht K, Gross H J
机构信息
Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Würzburg, F.R.G.
出版信息
Gene. 1987;60(1):137-44. doi: 10.1016/0378-1119(87)90221-6.
Screening of a human genomic DNA library with ribosomal 5S RNA yielded a variant 5S rRNA gene (pH5S1) and a pseudogene lacking the first 9 bp and the last 33 bp (pH5S2). Sequence analysis revealed that both genes contain several mutations in their coding region as compared to human 5S rRNA; however, their intragenic promoters are highly conserved. Both genes are transcribed in a homologous HeLa cell S100 extract. pH5S1 gives rise to a 5S-sized product, whereas the two pH5S2-derived RNAs are about 220 and 240 nucleotides long. pH5S1 is transcribed more efficiently than pH5S2; however, its ability to form a stable preinitiation complex is impaired.
用核糖体5S RNA筛选人类基因组DNA文库，得到一个变异的5S rRNA基因（pH5S1）和一个缺失前9个碱基对和后33个碱基对的假基因（pH5S2）。序列分析表明，与人类5S rRNA相比，这两个基因在其编码区都含有多个突变；然而，它们的基因内启动子高度保守。这两个基因都在同源的HeLa细胞S100提取物中转录。pH5S1产生一个5S大小的产物，而两个来自pH5S2的RNA长度约为220和240个核苷酸。pH5S1的转录效率比pH5S2高；然而，其形成稳定起始前复合物的能力受损。
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