Yan Xinlei, Miao Jing, Zhang Bao, Liu Huan, Ma Huifang, Sun Yufei, Liu Pufang, Zhang Xiujuan, Wang Ruigang, Kan Juntao, Yang Feiyun, Wu Qiming
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, China.
The Institute of Biotechnology, Inner Mongolia Academy of Science and Technology, Hohhot, China.
Front Nutr. 2023 Jul 17;10:1201919. doi: 10.3389/fnut.2023.1201919. eCollection 2023.
() is a homologous plant with high medicinal and edible value. Therefore, the extraction methods of Astragalus polysaccharide (APS) have attracted the attention of many research groups, but the yield of the active components is still not high. The aim of this study was to extract APS by a semi-bionic extraction method, optimize the extraction process, and evaluate the anti-aging activities of APS . The results showed that the APS yield was 18.23% when extracted by the semi-bionic extraction method. Anti-aging evaluation in rats showed that APS extracted by this method significantly decreased the malondialdehyde (MDA) content and increased superoxide dismutase (SOD) activity to cope with D-galactose-induced aging. Serum metabolomic analysis indicated that a total of 48 potential biomarkers showed significant differences, mainly involving 5 metabolic pathways. These altered metabolic pathways were mainly related to energy metabolism, amino acid metabolism, and lipid metabolism. These results indicated that the semi-bionic extraction method can effectively improve the yield of APS, and the extracted APS exhibited anti-aging activity in rats. Our study provided a novel and effective method to extract APS and indicated that APS can be used as functional food and natural medicine to delay aging and prevent its complications.
（）是一种具有高药用和食用价值的同源植物。因此，黄芪多糖（APS）的提取方法引起了许多研究团队的关注，但活性成分的产量仍然不高。本研究的目的是采用半仿生提取法提取APS，优化提取工艺，并评价APS的抗衰老活性。结果表明，采用半仿生提取法提取时，APS的得率为18.23%。对大鼠的抗衰老评价表明，用该方法提取的APS显著降低了丙二醛（MDA）含量，提高了超氧化物歧化酶（SOD）活性，以应对D-半乳糖诱导的衰老。血清代谢组学分析表明，共有48种潜在生物标志物存在显著差异，主要涉及5条代谢途径。这些改变的代谢途径主要与能量代谢、氨基酸代谢和脂质代谢有关。这些结果表明，半仿生提取法能有效提高APS的得率，且提取的APS在大鼠中表现出抗衰老活性。我们的研究提供了一种新颖有效的提取APS的方法，并表明APS可用作功能性食品和天然药物来延缓衰老并预防其并发症。
