Gatrill A J, Mackenzie C D, McMahon J E, Williams J F, Guderian R H
Wolfson Tropical Pathology Unit, London School of Hygiene and Tropical Medicine, UK.
Histochem J. 1987 Sep;19(9):509-19. doi: 10.1007/BF01675422.
Immunocytochemical and histochemical properties of macrophages present in the subcutaneous chronic inflammatory responses surrounding adult Onchocerca volvulus (nodules) in human tissues were examined. Macrophages with strong non-specific esterase (NSE) and acid phosphatase (AcPase) activities but weak adenosine triphosphatase (ATPase) activity and HLA-DR expression (NSE+++, AcPase+++, ATPase-/+, HLA-DR-/+) were present in the centre of nodules. Many of the cells adhering to the surface of worms were NSE+++, AcPase+++, ATPase-, HLA-DR+++. The inner zone of the fibrous capsule of nodules contained macrophages with the profile NSE+++, AcPase-, ATPase-/+, HLA-DR-/+. A fourth type, NSE+++, AcPase-/+, ATPase-/+, HLA-DR+++, was located in the outer zone of the capsule, frequently within perivascular accumulations of macrophages, lymphocytes and plasma cells. Active fibroblasts were identified at the inner edge of the fibrous capsule by alkaline phosphatase staining. A feature of all nodules examined was the presence of lipid-filled macrophages, demonstrated by Oil Red O stain; these cells were usually situated in zones adjacent to the centre of nodules, and were of the NSE++, AcPase++, ATPase-/+, HLA-DR-/+ type. Lipid accumulation was not found to be related to the clinical status of the patients studied. The origin and functional significance of this lipid is unknown.
对人体组织中成年盘尾丝虫（结节）周围皮下慢性炎症反应中存在的巨噬细胞的免疫细胞化学和组织化学特性进行了研究。在结节中心存在具有强非特异性酯酶（NSE）和酸性磷酸酶（AcPase）活性但腺苷三磷酸酶（ATPase）活性弱且HLA - DR表达弱的巨噬细胞（NSE+++，AcPase+++，ATPase - / +，HLA - DR - / +）。许多附着在虫体表面的细胞为NSE+++，AcPase+++，ATPase - ，HLA - DR+++。结节纤维囊的内层含有具有NSE+++，AcPase - ，ATPase - / +，HLA - DR - / +特征的巨噬细胞。第四种类型，NSE+++，AcPase - / +，ATPase - / +，HLA - DR+++，位于囊的外层，常存在于巨噬细胞、淋巴细胞和浆细胞的血管周围聚集体内。通过碱性磷酸酶染色在纤维囊的内边缘鉴定出活跃的成纤维细胞。所有检查的结节的一个特征是存在用油红O染色显示的充满脂质的巨噬细胞；这些细胞通常位于与结节中心相邻的区域，属于NSE++，AcPase++，ATPase - / +，HLA - DR - / +类型。未发现脂质积累与所研究患者的临床状况相关。这种脂质的来源和功能意义尚不清楚。
