Selby W S, Poulter L W, Hobbs S, Jewell D P, Janossy G
J Clin Pathol. 1983 Apr;36(4):379-84. doi: 10.1136/jcp.36.4.379.
HLA-DR-positive histiocytes in the lamina propria of the human intestine have been characterised using combined histochemical and immunohistological techniques. In the small intestine, 80-90% of the HLA-DR+ histiocytes had irregular surfaces with stellate processes, and exhibited strong membrane adenosine triphosphatase (ATPase) activity, but weak acid phosphatase (ACP) and non-specific esterase (NSE) activities (HLA-DR+ ACP+/- NSA+/- ATP++; type 1 cell). In contrast, in the lamina propria of the colon the majority (60-70%) of HLA-DR+ cells were large, round cells with strong ACP and NSE activities but no detectable ATPase activity (HLA-DR+ ACP++ NSE++ ATP+/-; type 2 cell). The colon also contained a population of type 1 cells (30-40%). In active inflammatory bowel disease affecting the colon a third population of HLA-DR+ histiocytes was seen. These cells were irregular in outline, with many processes, and were ACP++ NSE+ ATP+/- (type 3 cell). The type 3 cells appeared to replace type 2 cells. After treatment, the appearances returned to normal. These findings suggest that the different populations of HLA-DR+ histiocytes in the human intestine may have several functions, reflecting the different forms of antigen present in the intestine. The alterations in inflammatory bowel disease may represent activation in response to an invading antigen.
利用组织化学和免疫组织学相结合的技术，对人肠道固有层中的HLA - DR阳性组织细胞进行了特征描述。在小肠中，80 - 90%的HLA - DR +组织细胞表面不规则，有星状突起，表现出强烈的膜三磷酸腺苷酶（ATPase）活性，但酸性磷酸酶（ACP）和非特异性酯酶（NSE）活性较弱（HLA - DR + ACP + / - NSA + / - ATP ++；1型细胞）。相比之下，在结肠固有层中，大多数（60 - 70%）HLA - DR +细胞是大的圆形细胞，具有强烈的ACP和NSE活性，但未检测到ATPase活性（HLA - DR + ACP ++ NSE ++ ATP + / -；2型细胞）。结肠中也含有一群1型细胞（30 - 40%）。在影响结肠的活动性炎症性肠病中，可见到第三群HLA - DR +组织细胞。这些细胞轮廓不规则，有许多突起，ACP ++ NSE + ATP + / -（3型细胞）。3型细胞似乎取代了2型细胞。治疗后，外观恢复正常。这些发现表明，人肠道中不同群体的HLA - DR +组织细胞可能具有多种功能，反映了肠道中存在的不同形式的抗原。炎症性肠病中的改变可能代表对入侵抗原的反应性激活。
