Unit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma, Rome, Italy.
Department of Human Sciences and Quality of Life Promotion, San Raffaele University, Unit of Clinical and Molecular Epidemiology, IRCCS San Raffaele Roma, Via di Val Cannuta, 247, 00166, Rome, Italy.
Sci Rep. 2021 Aug 18;11(1):16793. doi: 10.1038/s41598-021-95976-7.
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan-Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06-1.90) for overall mortality, and 1.94 (1.04-3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
彗星试验或单细胞凝胶电泳是最常用于测量人群中链断裂和各种其他 DNA 损伤的方法。为了估计与 DNA 损伤相关的总死亡率、死因死亡率和癌症发病率的风险，我们对 1996 年至 2016 年间在 16 个实验室使用彗星试验进行筛查的 2403 名健康个体（25978 人年）进行了随访。卡普兰-迈耶分析表明，DNA 损伤中值和高值 tertile 的总生存率较差（p<0.001）。根据 Cox 比例风险回归模型对 DNA 损伤对生存的影响进行了建模。在 DNA 损伤最高 tertile 的个体中，总死亡率的调整后危险比（HR）为 1.42（1.06-1.90），循环系统疾病的 HR 为 1.94（1.04-3.59）。本研究的结果提供了流行病学证据，鼓励在预防非传染性疾病的策略中实施彗星试验。
