Diem Elisabeth, Ivancsits Sabine, Rüdiger Hugo W
Department of Occupational Medicine, University of Vienna, Austria.
J Toxicol Environ Health A. 2002 May 10;65(9):641-8. doi: 10.1080/15287390252900331.
In order to determine background levels of DNA strand breaks, we examined 80 healthy individuals by comet assay considering age, sex, and smoking as confounding factors. Only age was found to have a significant effect on basal levels. One thousand cells of each donor were graded by eye into 5 categories according to the amount of DNA in the tail: classification group A (no damage) <5%, B (low damage) 5-20%, C (medium damage) 20-40%, D (high damage) 40-95%, and group E (total damage) >95%. The interpretation of the comet assay was modified to achieve a tail factor, which represents the DNA damage of 1000 scored cells as a single number, without the need of an image analysis software package. Hydrogen peroxide and bleomycin used for in vitro exposure of lymphocytes, produced clear dose-related responses in the comet assay. Our data encourage the application of the used classification model for a sensitive and fast quantification of DNA damage. Results in this study are in agreement with most of the earlier investigations.
为了确定DNA链断裂的背景水平，我们通过彗星试验检查了80名健康个体，将年龄、性别和吸烟视为混杂因素。仅发现年龄对基础水平有显著影响。根据尾部DNA的量，将每个供体的1000个细胞目测分为5类：分类组A（无损伤）<5%，B（低损伤）5-20%，C（中度损伤）20-40%，D（高度损伤）40-95%，E组（完全损伤）>95%。对彗星试验的解释进行了修改，以获得一个尾部因子，该因子将1000个计分细胞的DNA损伤表示为一个单一数字，无需图像分析软件包。用于淋巴细胞体外暴露的过氧化氢和博来霉素在彗星试验中产生了明显的剂量相关反应。我们的数据鼓励应用所使用的分类模型对DNA损伤进行敏感和快速的定量。本研究的结果与大多数早期调查结果一致。
