Chakrabarty A N, Dastidar S G, Das S, Chaudhury S K
Deptt. of Medical Microbiology & Parasitology, University College of Medicine, Calcutta University.
Indian J Lepr. 1987 Jul-Sep;59(3):247-62.
Nocardia like organisms were isolated from all the 22 multibacillary cases of leprosy, on minimal media consisting of only mineral salts and supplemented with simple C-sources (e.g., liquid paraffin, tetradecane etc.) and N-sources (e.g. ammonium salts, urea, asparagine, gelatin etc.). Complex organic substances, e.g., xanthine, tyrosine, casein, peptone, meat extract, egg proteins, serum, blood, yeast extract as well as medium 199, did not support the growth of these organisms at all. Paraffin-urea minimal (PUM), paraffin gelatin minimal (PGM) and gelatin minimal medium, as well as the agar slants of these media, selectively allowed good growth of these organisms on which these could be serially propagated continuously, and isolated as pure cultures; these were acid-fast long slender rods which were seen to arise directly from fragmented or unfragmented long, slender hyphae, forming at places mycelial tufts many of which, on ageing, sporulated abundantly. Their acid-fastness was pyridine susceptible and these were DOPA-Oxidase positive; these grew best under reduced 02 tension, at pH 7.0-8.0 and temperature about 28 degrees C. Serologically, these appeared to be sufficiently related to, each other, 2 nocardiae (N. brasiliensis and N. caviae) and some mycobacteria.
在仅由无机盐组成、添加简单碳源（如液体石蜡、十四烷等）和氮源（如铵盐、尿素、天冬酰胺、明胶等）的基础培养基上，从22例多菌型麻风病例中均分离出诺卡菌样微生物。复杂有机物质，如黄嘌呤、酪氨酸、酪蛋白、蛋白胨、肉浸液、鸡蛋蛋白、血清、血液、酵母浸膏以及培养基199，根本不支持这些微生物生长。石蜡 - 尿素基础培养基（PUM）、石蜡明胶基础培养基（PGM）和明胶基础培养基，以及这些培养基的琼脂斜面，选择性地使这些微生物良好生长，在其上可连续传代培养，并分离为纯培养物；这些是抗酸的细长杆菌，可见它们直接从断裂或未断裂的长而细的菌丝产生，在某些部位形成菌丝丛，其中许多在老化时大量形成孢子。它们的抗酸性对吡啶敏感，且这些是多巴氧化酶阳性；它们在降低的氧张力、pH 7.0 - 8.0和温度约28℃的条件下生长最佳。从血清学上看，这些微生物彼此之间似乎与两种诺卡菌（巴西诺卡菌和豚鼠诺卡菌）以及一些分枝杆菌有充分的相关性。
