Department of Biology, Faculty of Science and Literature, Adıyaman University, Adiyaman, Turkey.
Department of Biology, Faculty of Science and Literature, Cukurova University, Adana, Turkey.
Biometals. 2021 Dec;34(6):1263-1273. doi: 10.1007/s10534-021-00341-w. Epub 2021 Aug 19.
The objective of the present study was to investigate the impact of zinc and copper on some biomarkers in a model organism Galleria mellonella L. We investigated the effects of Cu and Zn (10, 50, and 100 mg/100 g diets) on different biomarkers such as oxidative stress parameters (SOD and CAT activities and MDA levels), energy resources (protein, lipid and glycogen levels), electrolyte contents (Ca, Na, and K levels), total hemocyte count (THC), and growth and development of G. mellonella. Additionally, the accumulation levels of the used metals were also studied. Cu caused a significant decrease in protein, lipid and glycogen levels. SOD and CAT activities significantly increased at all concentrations of Cu, while they significantly increased at only high concentrations of Zn (50 and 100 mg). Lipid peroxidation levels (MDA) significantly elevated at high concentrations of both metals. It was determined that the Cu and Zn accumulation increased depending on the increase of the concentration. Zn caused an alteration in Ca level at the concentrations of 50 and 100 mg, and K and Na levels at all concentrations. While, THC significantly reduced at all Cu concentrations, this reduction was observed only at higher Zn concentrations (50 and 100 mg). Larval and pupal development time significantly extended at the highest concentration (100 mg) of Cu, and females' lifespan significantly shortened at all concentrations of Cu. Zinc caused an extension in larval development time at the highest concentration (100 mg), and caused a shortening in females and males' lifetime at all concentrations. The observed changes in biomarkers can be used as the illustration of potential toxic effects of high levels of Cu and Zn in organisms.
本研究的目的是研究锌和铜对模式生物家蚕某些生物标志物的影响。我们研究了 Cu 和 Zn（饮食中 10、50 和 100 mg/100 g）对不同生物标志物的影响，如氧化应激参数（SOD 和 CAT 活性和 MDA 水平）、能量资源（蛋白质、脂质和糖原水平）、电解质含量（Ca、Na 和 K 水平）、总血细胞计数（THC）以及家蚕的生长和发育。此外，还研究了所用金属的积累水平。Cu 导致蛋白质、脂质和糖原水平显著降低。SOD 和 CAT 活性在 Cu 的所有浓度下均显著增加，而仅在 Zn 的高浓度（50 和 100 mg）下才显著增加。脂质过氧化水平（MDA）在两种金属的高浓度下均显著升高。结果表明，Cu 和 Zn 的积累随着浓度的增加而增加。Zn 在 50 和 100 mg 的浓度下引起 Ca 水平的改变，以及所有浓度下的 K 和 Na 水平的改变。然而，在所有 Cu 浓度下，THC 显著降低，而这种降低仅在较高 Zn 浓度（50 和 100 mg）下观察到。幼虫和蛹的发育时间在 Cu 的最高浓度（100 mg）下显著延长，而在所有 Cu 浓度下雌性寿命显著缩短。Zn 导致最高浓度（100 mg）下幼虫发育时间延长，并导致所有浓度下雌性和雄性寿命缩短。生物标志物的观察到的变化可用作高浓度 Cu 和 Zn 对生物体潜在毒性影响的说明。
