Ding Chunjin, He Rundong, Zhang Jinghan, Dong Zhan, Wu Jun
Department of Orthopaedics, Affiliated Haian Hospital of Nantong University, Nantong, Jiangsu, China.
The Research Center for Bone and Stem Cells, Department of Anatomy, Histology and Embryology, Nanjing Medical University, Nanjing, Jiangsu, China.
Cancer Cell Int. 2021 Aug 19;21(1):435. doi: 10.1186/s12935-021-02141-1.
Pseudogenes played important roles in tumorigenesis, while there are nearly no reports about the expression and roles of HSPA7 in the cancer.
Firstly, we used Logistic regression, the KS test, the GEPIA database, UALCAN database and qRT-PCR to analyze the expression level of HSPA7 in KIRC, then we used the Cox regression and the Kaplan-Meier curve to analyze the overall survival (OS) of KIRC patients with different Clinico-pathological parameters. Thirdly, we used the multivariate Cox analysis of influencing factors to compare the correlation between the HSPA7 expression level and the clinical parameters. Finally, we used multi-GSEA analysis and the Tumor Immunoassay Resource (TIMER) database to explore the functional role of HSPA7 in KIRC RESULTS: The HSPA7 is highly expressed in KIRC tumor tissues, and its expression is related to clinico-pathological features and survival in KIRC patients. GSEA analysis displayed the high expression of HSPA7 in KIRC were related to several tumor-related and immune-related pathways. With the TIMER database analysis we showed that HSPA7 levels were correlated with the CD4 T cells, neutrophils and Dendritic Cell.
Our study showed that HSPA7 is very important in the tumor progression and may act as a poor prognostic biomarker for KIRC tumor by modulating immune infiltrating cells.
假基因在肿瘤发生中起重要作用，而关于HSPA7在癌症中的表达及作用的报道几乎没有。
首先，我们使用逻辑回归、KS检验、GEPIA数据库、UALCAN数据库和qRT-PCR分析KIRC中HSPA7的表达水平，然后使用Cox回归和Kaplan-Meier曲线分析不同临床病理参数的KIRC患者的总生存期（OS）。第三，我们使用影响因素的多变量Cox分析来比较HSPA7表达水平与临床参数之间的相关性。最后，我们使用多GSEA分析和肿瘤免疫测定资源（TIMER）数据库来探索HSPA7在KIRC中的功能作用。结果：HSPA7在KIRC肿瘤组织中高表达，其表达与KIRC患者的临床病理特征和生存相关。GSEA分析显示KIRC中HSPA7的高表达与几种肿瘤相关和免疫相关途径有关。通过TIMER数据库分析，我们表明HSPA7水平与CD4 T细胞、中性粒细胞和树突状细胞相关。
我们的研究表明，HSPA7在肿瘤进展中非常重要，可能通过调节免疫浸润细胞作为KIRC肿瘤预后不良的生物标志物。
