易剪（Easy-Prime）：一款基于机器学习的碱基编辑器设计工具。

Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, USA.

Integrated Biomedical Sciences Program, University of Tennessee Health Science Center, Memphis, TN, USA.

Genome Biol. 2021 Aug 19;22(1):235. doi: 10.1186/s13059-021-02458-0.

Prime editing is a revolutionary genome-editing technology that can make a wide range of precise edits in DNA. However, designing highly efficient prime editors (PEs) remains challenging. We develop Easy-Prime, a machine learning-based program trained with multiple published data sources. Easy-Prime captures both known and novel features, such as RNA folding structure, and optimizes feature combinations to improve editing efficiency. We provide optimized PE design for installation of 89.5% of 152,351 GWAS variants. Easy-Prime is available both as a command line tool and an interactive PE design server at: http://easy-prime.cc/ .

碱基编辑是一种革命性的基因组编辑技术，可以在 DNA 中实现广泛的精确编辑。然而，设计高效的碱基编辑器（PEs）仍然具有挑战性。我们开发了基于机器学习的程序 Easy-Prime，该程序经过多个已发表数据来源的训练。Easy-Prime 捕获了已知和新颖的特征，如 RNA 折叠结构，并优化了特征组合以提高编辑效率。我们为安装 152351 个 GWAS 变体中的 89.5%提供了优化的 PE 设计。Easy-Prime 既可以作为命令行工具使用，也可以在交互式 PE 设计服务器上使用，网址为：http://easy-prime.cc/ 。