Jin Tao, Xu Yong, Dai Chenwei, Zhou Xiuhong, Xu Qinghua, Wu Zhengwei
School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, China.
Anhui Academy of Medical Sciences, Hefei, China.
AIP Adv. 2021 Aug 10;11(8):085019. doi: 10.1063/5.0060530. eCollection 2021 Aug.
Cold atmospheric plasma (CAP), regarded as a powerful physics technology, displays antimicrobial, antitumor, and even antiviral properties, but the underlying mechanism is rarely studied. In this study, four CAP exposure doses (30, 60, 120, and 240 s) were applied to inactivate a severe acute respiratory syndrome coronavirus 2 like pseudovirus on a stainless steel disk, which comprised spike protein on its membrane and can express a green fluorescent protein. In order to unravel the potential effects of CAP irradiation on pseudovirus, infection assay, optical emission spectra analysis, transmission electron microscopy (TEM), sodium dodecyl sulfate polyacrylamide gel electrophoresis, ELISA, and qPCR experiments were carried out. As a result, our study indicated that CAP irradiation can significantly decrease the infectivity of pseudovirus in a dose dependent manner through destroying the cell membrane and further damaging viral RNA, with the molecular weight and conformation of spike receptor binding domain protein unchanged.
冷大气等离子体（CAP）被视为一种强大的物理技术，具有抗菌、抗肿瘤甚至抗病毒特性，但其潜在机制鲜有研究。在本研究中，应用四种CAP暴露剂量（30、60、120和240秒）使不锈钢盘上的严重急性呼吸综合征冠状病毒2样假病毒失活，该假病毒在其膜上含有刺突蛋白并能表达绿色荧光蛋白。为了阐明CAP辐照对假病毒的潜在影响，进行了感染试验、发射光谱分析、透射电子显微镜（TEM）、十二烷基硫酸钠聚丙烯酰胺凝胶电泳、酶联免疫吸附测定（ELISA）和定量聚合酶链反应（qPCR）实验。结果表明，CAP辐照可通过破坏细胞膜并进一步损伤病毒RNA，以剂量依赖的方式显著降低假病毒的感染性，而刺突受体结合域蛋白的分子量和构象未发生变化。
