Doria G, Agarossi G, Boraschi D, Antonietta M
Immunology. 1977 Apr;32(4):539-48.
The effect of carrier priming on antibody avidity was investigated under several experimental conditions. Basically, mice were carrier primed with HRBC (horse red blood cells) prior to immunization with TNP (2,4,6-trinitrophenyl) conjugated to HRBC. Immunization was performed either or in spleen cell culture, and avidity of anti-TNP antibodies was estimated from inhibition of direct PFC (plaque-forming cells) by free TNP-BSA (-bovine serum albumin). The data indicate the appropriate conditions under which carrier priming can enhance antibody avidity. The carrier effect is maximized by priming the animals with 10-10 HRBC 3-7 days before immunization with a low dose of TNP-HRBC. Hyper-immunization by repeated injections of a high dose of the conjugate does not modify the carrier effect on avidity but it delays the fall of avidity in both carrier primed and unprimed animals. These results are interpreted in terms of T- and B-cell co-operation within the framework of the maturation theory of antibody affinity. Carrier priming was also found to increase the number of direct PFC of the IgM and, mostly, of the non-IgM classes, a finding in agreement with the notion that T cells can help IgM production and the shift to IgG.
在几种实验条件下研究了载体致敏对抗体亲和力的影响。基本上，在用与HRBC（马红细胞）偶联的TNP（2,4,6-三硝基苯基）免疫之前，先用HRBC对小鼠进行载体致敏。免疫在体内或脾细胞培养中进行，通过游离TNP-BSA（牛血清白蛋白）对直接PFC（空斑形成细胞）的抑制作用来估计抗TNP抗体的亲和力。数据表明了载体致敏可增强抗体亲和力的适宜条件。在用低剂量TNP-HRBC免疫前3至7天用10⁷-10⁸个HRBC对动物进行致敏，可使载体效应最大化。通过重复注射高剂量偶联物进行超免疫不会改变载体对亲和力的影响，但会延迟载体致敏和未致敏动物中亲和力的下降。这些结果根据抗体亲和力成熟理论框架内的T细胞和B细胞合作进行解释。还发现载体致敏可增加IgM类尤其是非IgM类直接PFC的数量，这一发现与T细胞可帮助IgM产生并向IgG转变的观点一致。
