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RanGTP 和肌动蛋白细胞骨架在受精过程中使父本和母本染色体分离。

RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization.

机构信息

Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.

Laboratory for Chromosome Segregation, RIKEN Center for Biosystems Dynamics Research, Kobe, Japan.

出版信息

J Cell Biol. 2021 Oct 4;220(10). doi: 10.1083/jcb.202012001. Epub 2021 Aug 23.

DOI:10.1083/jcb.202012001
PMID:34424312
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8404465/
Abstract

Zygotes require two accurate sets of parental chromosomes, one each from the mother and the father, to undergo normal embryogenesis. However, upon egg-sperm fusion in vertebrates, the zygote has three sets of chromosomes, one from the sperm and two from the egg. The zygote therefore eliminates one set of maternal chromosomes (but not the paternal chromosomes) into the polar body through meiosis, but how the paternal chromosomes are protected from maternal meiosis has been unclear. Here we report that RanGTP and F-actin dynamics prevent egg-sperm fusion in proximity to maternal chromosomes. RanGTP prevents the localization of Juno and CD9, egg membrane proteins that mediate sperm fusion, at the cell surface in proximity to maternal chromosomes. Following egg-sperm fusion, F-actin keeps paternal chromosomes away from maternal chromosomes. Disruption of these mechanisms causes the elimination of paternal chromosomes during maternal meiosis. This study reveals a novel critical mechanism that prevents aneuploidy in zygotes.

摘要

受精卵需要来自父母双方的两套准确的染色体，一套来自母亲，一套来自父亲，才能正常进行胚胎发生。然而，在脊椎动物的卵子-精子融合过程中，受精卵有三套染色体，一套来自精子，两套来自卵子。因此，受精卵通过减数分裂将一套来自母体的染色体（而不是来自父体的染色体）排入极体，但父体染色体如何免受母体减数分裂的影响尚不清楚。在这里，我们报告说，RanGTP 和 F-肌动蛋白动力学阻止了靠近母体染色体的卵子-精子融合。RanGTP 阻止了 Juno 和 CD9（介导精子融合的卵子膜蛋白）在靠近母体染色体的细胞表面的定位。卵子-精子融合后，F-肌动蛋白使父体染色体远离母体染色体。破坏这些机制会导致在母体减数分裂过程中消除父体染色体。这项研究揭示了防止受精卵非整倍体的一种新的关键机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/a7cbe47e0dc2/JCB_202012001_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/2a92636c3eb1/JCB_202012001_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/dc15fe721524/JCB_202012001_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/53b0942d093b/JCB_202012001_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/4a9c8455607c/JCB_202012001_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/d5446e9ce4d1/JCB_202012001_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/fab81e95a8bc/JCB_202012001_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/4429b2311bdc/JCB_202012001_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/b8cc257bd311/JCB_202012001_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/8c622d18e37a/JCB_202012001_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/99e818a0e786/JCB_202012001_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/2d4da4c95d98/JCB_202012001_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/0974beab224f/JCB_202012001_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/a7cbe47e0dc2/JCB_202012001_Fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/2a92636c3eb1/JCB_202012001_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/dc15fe721524/JCB_202012001_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/53b0942d093b/JCB_202012001_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/4a9c8455607c/JCB_202012001_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/d5446e9ce4d1/JCB_202012001_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/fab81e95a8bc/JCB_202012001_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/4429b2311bdc/JCB_202012001_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/b8cc257bd311/JCB_202012001_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/8c622d18e37a/JCB_202012001_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/99e818a0e786/JCB_202012001_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/2d4da4c95d98/JCB_202012001_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/0974beab224f/JCB_202012001_Fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9702/8404465/a7cbe47e0dc2/JCB_202012001_Fig8.jpg

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