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一种富含细胞质的环状RNA circ-MYBL2在非小细胞肺癌中表达下调，并通过海绵吸附致癌性miR-28来调节癌细胞的增殖和凋亡。

A Cytoplasm-Enriched circRNA circ-MYBL2 is Downregulated in Non-Small Cell Lung Cancer and Sponges Oncogenic miR-28 to Regulate Cancer Cell Proliferation and Apoptosis.

作者信息

Mao Yanqing, Wang Chunjie

机构信息

Department of General Practice, Dushu Lake Hospital Affiliated to Soochow University, Suzhou, 215000, People's Republic of China.

Department of General Practice, The First Affiliated Hospital of Soochow University, Suzhou, 215000, People's Republic of China.

出版信息

Cancer Manag Res. 2021 Aug 16;13:6499-6506. doi: 10.2147/CMAR.S309924. eCollection 2021.

DOI:10.2147/CMAR.S309924
PMID:34429656
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8379391/
Abstract

BACKGROUND

Recent studies have reported different roles of circRNA circ-MYBL2 in different cancers. However, the involvement of circ-MYBL2 in non-small cell lung cancer (NSCLC) is unknown. This study was carried out to explore the role of circ-MYBL2 in NSCLC.

METHODS

The expression of circ-MYBL2 and miR-28 was detected by RT-qPCR. A 5-year follow-up study was performed for survival analysis. Nuclear fractionation assay was used for subcellular localization analysis. RNA pull-down assay was performed to detect the interaction between circ-MYBL2 and miR-28. The role of circ-MYBL2 and miR-28 in regulating the expression of each other was evaluated by overexpression assay. BrdU incorporation assay and cell apoptosis assay were performed to investigate the role of circ-MYBL2 and miR-28 in cell proliferation and apoptosis.

RESULTS

NSCLC tissues exhibited significantly higher expression levels of miR-28 and lower expression levels of circ-MYBL2. Close correlations between circ-MYBL2 and miR-28 and patients' survival were observed. Circ-MYBL2, which was found to be mainly enriched in cytoplasm, directly interacted with miR-28. Although circ-MYBL2 and miR-28 showed no regulatory role in the expression of each other, circ-MYBL2 suppressed the effects of miR-28 on cell proliferation and apoptosis.

CONCLUSION

Circ-MYBL2 is enriched in cytoplasm, and it sponges oncogenic miR-28 to suppress cancer cell proliferation in NSCLC and promote cell apoptosis.

摘要

背景

近期研究报道了环状RNA circ-MYBL2在不同癌症中的不同作用。然而，circ-MYBL2在非小细胞肺癌（NSCLC）中的作用尚不清楚。本研究旨在探讨circ-MYBL2在NSCLC中的作用。

方法

采用RT-qPCR检测circ-MYBL2和miR-28的表达。进行为期5年的随访研究以进行生存分析。采用细胞核分级分离试验进行亚细胞定位分析。进行RNA下拉试验以检测circ-MYBL2与miR-28之间的相互作用。通过过表达试验评估circ-MYBL2和miR-28在调节彼此表达中的作用。进行BrdU掺入试验和细胞凋亡试验以研究circ-MYBL2和miR-28在细胞增殖和凋亡中的作用。

结果

NSCLC组织中miR-28表达水平显著升高，circ-MYBL2表达水平降低。观察到circ-MYBL2与miR-28之间以及与患者生存率之间存在密切相关性。发现circ-MYBL2主要富集于细胞质中，可直接与miR-28相互作用。虽然circ-MYBL2和miR-28在彼此的表达中未显示出调节作用，但circ-MYBL2可抑制miR-28对细胞增殖和凋亡的影响。

结论

Circ-MYBL2富集于细胞质中，它可吸附致癌性miR-28，从而抑制NSCLC中的癌细胞增殖并促进细胞凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/8fc34b6c1e07/CMAR-13-6499-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/8f06feaed201/CMAR-13-6499-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/936075a28a82/CMAR-13-6499-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/74f57e1d6113/CMAR-13-6499-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/8fc34b6c1e07/CMAR-13-6499-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/8f06feaed201/CMAR-13-6499-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/936075a28a82/CMAR-13-6499-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/74f57e1d6113/CMAR-13-6499-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b59d/8379391/8fc34b6c1e07/CMAR-13-6499-g0004.jpg

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