Cornelis G, Luke R K, Richmond M H
J Clin Microbiol. 1978 Feb;7(2):180-3. doi: 10.1128/jcm.7.2.180-183.1978.
Introduction of plasmids carrying the lacY gene (lactose permease gene) into Yersinia enterocolitica results in cells being able to ferment both lactose and raffinose. Transfer of such plasmids into Escherichia coli C600 (lacY) confers ability to ferment lactose but not raffinose. Derivatives of C600 that ferment both lactose and sucrose (Lac+ Scr+ strains) are able to ferment raffinose, but do not grow well on raffinose minimal medium. Fermentation of raffinose by Lac+ strains of Y. enterocolitica, and by Lac+ Scr+ strains of E. coli, is explained in terms of transport of raffinose via the lac permease and subsequent breakdown catalyzed by invertase.
将携带lacY基因(乳糖通透酶基因)的质粒导入小肠结肠炎耶尔森菌,会使细胞能够发酵乳糖和棉子糖。将此类质粒转入大肠杆菌C600(lacY),赋予其发酵乳糖的能力,但不能发酵棉子糖。能够发酵乳糖和蔗糖的C600衍生物(Lac+ Scr+菌株)能够发酵棉子糖,但在棉子糖基本培养基上生长不佳。小肠结肠炎耶尔森菌的Lac+菌株以及大肠杆菌的Lac+ Scr+菌株对棉子糖的发酵,可通过棉子糖经乳糖通透酶转运以及随后由转化酶催化的分解来解释。