大肠杆菌K-12中缺乏α-半乳糖苷酶和硫代甲基半乳糖苷通透酶II的蜜二糖突变体分析。
Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12.
作者信息
Schmitt R
出版信息
J Bacteriol. 1968 Aug;96(2):462-71. doi: 10.1128/jb.96.2.462-471.1968.
Abstract
Three types of mutants (mel(-)) unable to metabolize the alpha-d-galactoside, melibiose, were derived from Escherichia coli K-12. One type lacked alpha-galactosidase; another lacked a specific transport system, termed thiomethylgalactoside (TMG) permease II; and the third lacked both of these functions. The mutational sites were genetically mapped by recombination frequency with different markers and by determination of chromosomal transfer in interrupted-mating experiments. All three mel(-) mutant types mapped in a cluster near to the metA marker on the E. coli chromosome and were cotransducible. Induction studies revealed that the three alpha-d-galactosides, melibiose, melibiitol, and galactinol, induced alpha-galactosidase and TMG permease II coordinately; d-galactose also induced them but only in a galactokinaseless mutant. These data suggest that alpha-galactosidase and TMG permease II may be components of a common operon.
摘要
从大肠杆菌K-12中获得了三种无法代谢α-D-半乳糖苷蜜二糖的突变体（mel(-)）。一种类型缺乏α-半乳糖苷酶；另一种缺乏一种特定的转运系统，称为硫代甲基半乳糖苷（TMG）通透酶II；第三种则同时缺乏这两种功能。通过与不同标记的重组频率以及在中断杂交实验中测定染色体转移，对突变位点进行了遗传定位。所有三种mel(-)突变体类型都定位在大肠杆菌染色体上靠近metA标记的一个簇中，并且是共转导的。诱导研究表明，三种α-D-半乳糖苷，蜜二糖、蜜二糖醇和棉子糖，协同诱导α-半乳糖苷酶和TMG通透酶II；D-半乳糖也能诱导它们，但仅在无半乳糖激酶的突变体中。这些数据表明，α-半乳糖苷酶和TMG通透酶II可能是一个共同操纵子的组成部分。
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本文引用的文献
1
Linkage Analysis with Very High Frequency Males of Escherichia Coli.大肠杆菌高频雄性菌株的连锁分析
Genetics. 1960 Sep;45(9):1233-43. doi: 10.1093/genetics/45.9.1233.
2
[Sexuality of bacteria].[细菌的性行为]
Boll Ist Sieroter Milan. 1950 Sep-Oct;29(9-10):281-9.
3
A SECOND PERMEASE FOR METHYL-THIO-BETA-D-GALACTOSIDE IN ESCHERICHIA COLI.大肠杆菌中甲基硫代-β-D-半乳糖苷的第二种通透酶
Biochim Biophys Acta. 1965 May 4;100:591-3. doi: 10.1016/0304-4165(65)90029-2.
4
THE GENETIC MAP OF ESCHERICHIA COLI K-12.大肠杆菌K-12的遗传图谱
Genetics. 1964 Oct;50(4):659-77. doi: 10.1093/genetics/50.4.659.
5
A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.大肠杆菌中乳糖操纵子区域的缺失分析
J Mol Biol. 1964 Mar;8:427-30. doi: 10.1016/s0022-2836(64)80206-0.
6
Recombination studies of lactose nonfermenting mutants of Escherichia coli K-12.大肠杆菌K-12乳糖非发酵突变体的重组研究。
Genetics. 1962 Oct;47(10):1335-53. doi: 10.1093/genetics/47.10.1335.
7
Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles.大肠杆菌和痢疾志贺氏菌菌株间乳糖利用能力的转导及转导噬菌体颗粒的特性
Virology. 1960 Nov;12:348-90. doi: 10.1016/0042-6822(60)90161-6.
8
Transport of galactose from the inside to the outside of Escherichia coli.半乳糖在大肠杆菌内从内部向外部的转运。
Pathol Biol. 1961 Apr;9:806-10.
9
Genetic regulatory mechanisms in the synthesis of proteins.蛋白质合成中的遗传调控机制。
J Mol Biol. 1961 Jun;3:318-56. doi: 10.1016/s0022-2836(61)80072-7.
10
Genetic and physical determinations of chromosomal segments in Escherichia coli.大肠杆菌染色体片段的遗传和物理测定
Symp Soc Exp Biol. 1958;12:75-92.
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