用于检测抗天然脱氧核糖核酸抗体的半自动预标准化免疫荧光检测系统的评估
Evaluation of a semiautomated prestandardized immunofluorescence test system for detection of anti-native deoxyribonucleic acid antibodies.
作者信息
Tourville D R, Benn V
出版信息
J Clin Microbiol. 1978 Feb;7(2):219-22. doi: 10.1128/jcm.7.2.219-222.1978.
An immunofluorescence-native deoxyribonucleic acid (nDNA) antibody test system (Zeus Scientific, Inc.) was compared with a radioimmunoassay procedure (FARR assay) for detecting anti-nDNA antibodies in human serum. Double-blind studies of split samples obtained from 236 patients showed an 80% correlation between the immunofluorescence-nDNA antibody test system and the radioimmunoassay procedure. Studies of sera from patients with known diagnoses showed positive nDNA antibody findings in biopsy-prove n systemic lupus erythematosus only. The immunofluorescence-nDNA antibody test system provides a reliable, simple, and economically feasible alternative method for detecting nDNA antibodies that can be employed in any laboratory equipped with a fluorescence microscope.
将一种免疫荧光天然脱氧核糖核酸（nDNA）抗体检测系统（宙斯科学公司）与一种放射免疫测定法（FARR测定法）进行比较，以检测人血清中的抗nDNA抗体。对从236名患者身上采集的分离样本进行的双盲研究表明，免疫荧光nDNA抗体检测系统与放射免疫测定法之间的相关性为80%。对已知诊断患者的血清研究表明，仅在经活检证实的系统性红斑狼疮患者中发现了阳性nDNA抗体结果。免疫荧光nDNA抗体检测系统为检测nDNA抗体提供了一种可靠、简单且经济可行的替代方法，可在任何配备荧光显微镜的实验室中使用。
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