• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

分形维数分析：一种用于分析集落形成单位的新工具。

Fractal dimension analysis: A new tool for analyzing colony-forming units.

作者信息

Nai Gisele Alborghetti, Martelli Cesar Alberto TALAvera, Medina Denis Aloísio Lopes, Oliveira Mayla Silva Cayres de, Caldeira Isadora Delfino, Henriques Bruno Carvalho, Portelinha Maria Júlia Schadeck, Eller Lizziane Kretli Winkelstroter, Marques Mariângela Esther Alencar

机构信息

Department of Pathology, Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700, 19050-680 Presidente Prudente, SP, Brazil.

Department of Surgery, Universidade do Oeste Paulista (UNOESTE), Rua José Bongiovani, 700, 19050-680 Presidente Prudente, SP, Brazil.

出版信息

MethodsX. 2021 Jan 12;8:101228. doi: 10.1016/j.mex.2021.101228. eCollection 2021.

DOI:10.1016/j.mex.2021.101228
PMID:34434751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8374233/
Abstract

The gold standard for quantifying bacteria both in routine diagnostics and in research is plating followed by count of colony-forming units (CFU). But, manual CFU counting on plates is time-consuming and subjective. We evaluated fractal dimension as a new methodology for evaluating CFU. Twenty fragments of expanded polytetrafluoroethylene (ePTFE) synthetic vascular prosthesis and 20 silicone prostheses were embedded in bacterial suspensions and incubated. The prostheses were then sown in solid culture medium and incubated for 48 h. Petri dishes were photographed and analyzed by fractal dimension. There was correlation between the number of CFU in manual counting and the fractal dimension analysis ( = 0.0001). We demonstrated that fractal dimension is a useful method for microbiological analyses in researches. It makes CFU analysis easier and faster and can be used regardless of the culture medium.•Petri dishes with different bacterial colonies were photographed with a digital camera under natural light.•The images were binarized and analyzed with ImageJ's "fractal dimension" tool.•Fractal dimension analysis showed to be a good tool for evaluating the amount of colony-forming unit.

摘要

在常规诊断和研究中，对细菌进行定量的金标准是平板接种，然后对菌落形成单位（CFU）进行计数。但是，在平板上手动计数CFU既耗时又主观。我们评估了分形维数作为评估CFU的一种新方法。将20片膨体聚四氟乙烯（ePTFE）人工血管假体和20片硅胶假体嵌入细菌悬液中并进行培养。然后将假体接种于固体培养基中，培养48小时。对培养皿进行拍照，并通过分形维数进行分析。手动计数的CFU数量与分形维数分析之间存在相关性（P = 0.0001）。我们证明分形维数是研究中微生物分析的一种有用方法。它使CFU分析更简便、快速，且无论使用何种培养基均可使用。

• 在自然光下用数码相机拍摄带有不同细菌菌落的培养皿。

• 对图像进行二值化处理，并使用ImageJ的“分形维数”工具进行分析。

• 分形维数分析显示是评估菌落形成单位数量的良好工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/e016fe4ef0b6/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/bbffd1f579e7/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/e4e1ff70fed8/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/1845e0ef56d4/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/b74010ba936e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/75ae4de42fdf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/2a46de6c2c56/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/e016fe4ef0b6/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/bbffd1f579e7/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/e4e1ff70fed8/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/1845e0ef56d4/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/b74010ba936e/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/75ae4de42fdf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/2a46de6c2c56/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8014/8374233/e016fe4ef0b6/gr6.jpg

相似文献

1
Fractal dimension analysis: A new tool for analyzing colony-forming units.分形维数分析：一种用于分析集落形成单位的新工具。
MethodsX. 2021 Jan 12;8:101228. doi: 10.1016/j.mex.2021.101228. eCollection 2021.
2
Automated image analysis with ImageJ of yeast colony forming units from cannabis flowers.使用ImageJ对大麻花朵中的酵母集落形成单位进行自动图像分析。
J Microbiol Methods. 2019 Sep;164:105681. doi: 10.1016/j.mimet.2019.105681. Epub 2019 Aug 2.
3
Label-free 1D microfluidic dipstick counting of microbial colonies and bacteriophage plaques.无标记 1D 微流控棍状条计数法用于微生物菌落和噬菌体噬菌斑的计数。
Lab Chip. 2022 Jul 26;22(15):2820-2831. doi: 10.1039/d2lc00280a.
4
Microliter spotting and micro-colony observation: A rapid and simple approach for counting bacterial colony forming units.微升点样和微菌落观察：一种快速简便的细菌菌落形成单位计数方法。
J Microbiol Methods. 2023 Apr;207:106707. doi: 10.1016/j.mimet.2023.106707. Epub 2023 Mar 15.
5
Effective object recognition for automated counting of colonies in Petri dishes (automated colony counting).用于培养皿中菌落自动计数（自动菌落计数）的有效目标识别
Comput Methods Programs Biomed. 2001 Sep;66(2-3):183-98. doi: 10.1016/s0169-2607(00)00128-0.
6
A cost-effective method for the automatic quantitative analysis of fibroblastic colony-forming units.一种用于成纤维细胞集落形成单位自动定量分析的经济高效方法。
Calcif Tissue Int. 1999 Aug;65(2):166-72. doi: 10.1007/s002239900677.
7
Automated counting of bacterial colony forming units on agar plates.琼脂平板上细菌菌落形成单位的自动计数。
PLoS One. 2012;7(3):e33695. doi: 10.1371/journal.pone.0033695. Epub 2012 Mar 20.
8
Machine learning for enumeration of cell colony forming units.用于细胞集落形成单位计数的机器学习
Vis Comput Ind Biomed Art. 2022 Nov 5;5(1):26. doi: 10.1186/s42492-022-00122-3.
9
An objective method to measure cell survival by computer-assisted image processing of numeric images of Petri dishes.一种通过对培养皿数字图像进行计算机辅助图像处理来测量细胞存活率的客观方法。
Phys Med Biol. 2003 Jun 7;48(11):1551-63. doi: 10.1088/0031-9155/48/11/305.
10
Automated counting of bacterial colonies on agar plates based on images captured at near-infrared light.基于近红外光下拍摄的图像对琼脂平板上的细菌菌落进行自动计数。
J Microbiol Methods. 2018 Oct;153:66-73. doi: 10.1016/j.mimet.2018.09.004. Epub 2018 Sep 6.

本文引用的文献

1
Automated image analysis with ImageJ of yeast colony forming units from cannabis flowers.使用ImageJ对大麻花朵中的酵母集落形成单位进行自动图像分析。
J Microbiol Methods. 2019 Sep;164:105681. doi: 10.1016/j.mimet.2019.105681. Epub 2019 Aug 2.
2
High-Throughput Quantification of Bacterial-Cell Interactions Using Virtual Colony Counts.利用虚拟集落计数高通量量化细菌-细胞相互作用。
Front Cell Infect Microbiol. 2018 Feb 15;8:43. doi: 10.3389/fcimb.2018.00043. eCollection 2018.
3
Fractal Dimension in Quantifying Experimental-Pulmonary-Hypertension-Induced Cardiac Dysfunction in Rats.
分形维数在量化大鼠实验性肺动脉高压所致心脏功能障碍中的应用
Arq Bras Cardiol. 2016 Jul;107(1):33-9. doi: 10.5935/abc.20160083. Epub 2016 May 24.
4
Fractal dimension of apical dendritic arborization differs in the superficial and the deep pyramidal neurons of the rat cerebral neocortex.大鼠大脑新皮质浅层和深层锥体神经元顶端树突分支的分形维数不同。
Neurosci Lett. 2015 Mar 4;589:88-91. doi: 10.1016/j.neulet.2015.01.044. Epub 2015 Jan 17.
5
Digital differentiation of non-small cell carcinomas of the lung by the fractal dimension of their epithelial architecture.
Micron. 2014 Dec;67:125-131. doi: 10.1016/j.micron.2014.07.005. Epub 2014 Jul 28.
6
NIH Image to ImageJ: 25 years of image analysis.NIH 图像到 ImageJ：25 年的图像分析。
Nat Methods. 2012 Jul;9(7):671-5. doi: 10.1038/nmeth.2089.
7
Glucose induced fractal colony pattern of Bacillus thuringiensis.葡萄糖诱导苏云金芽孢杆菌的分形菌落模式。
J Theor Biol. 2010 Aug 7;265(3):389-95. doi: 10.1016/j.jtbi.2010.05.016. Epub 2010 May 27.
8
A fractal analysis of pathogen detection by biosensors.生物传感器病原体检测的分形分析。
Biophys Chem. 2005 Jan 1;113(1):67-81. doi: 10.1016/j.bpc.2004.07.041.
9
Modeling spatio-temporal patterns generated by Bacillus subtilis.枯草芽孢杆菌产生的时空模式建模。
J Theor Biol. 1997 Sep 21;188(2):177-85. doi: 10.1006/jtbi.1997.0462.
10
Self-similar colony morphogenesis by gram-negative rods as the experimental model of fractal growth by a cell population.革兰氏阴性杆菌的自相似菌落形态发生作为细胞群体分形生长的实验模型。
Appl Environ Microbiol. 1992 Apr;58(4):1227-32. doi: 10.1128/aem.58.4.1227-1232.1992.