Institute of Microengineering and Nanoelectronics (IMEN), Universiti Kebangsaan Malaysia (UKM), Bangi 43600, Selangor, Malaysia.
Department of Chemistry, Faculty of Science, University Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Biosensors (Basel). 2021 Aug 7;11(8):267. doi: 10.3390/bios11080267.
The electrochemical biosensor devices based on enzymes for monitoring biochemical substances are still considered attractive. We investigated the immobilization of glucose oxidase (GOx) on a new composite nanomaterial poly(3,4-ethylenedioxythiophene): polystyrene sulfonate (PEDOT:PSS)/titanium carbide,(TiC)/graphene quantum dots(GQD) modified screen-printed carbon electrode (SPCE) for glucose sensing. The characterization and electrochemical behavior of PEDOT:PSS/TiC/GQD towards the electrocatalytic oxidation of GOx was analyzed by FTIR, XPS, SEM, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This composite nanomaterial was found to tend to increase the electrochemical behavior and led to a higher peak current of 100.17 µA compared to 82.01 µA and 95.04 µA for PEDOT:PSS and PEDOT:PSS/TiC alone. Moreover, the detection results demonstrated that the fabricated biosensor had a linear voltammetry response in the glucose concentration range 0-500 µM with a relatively sensitivity of 21.64 µAmMcm and a detection limit of 65 µM (S/N = 3), with good stability and selectivity. This finding could be useful as applicable guidance for the modification screen printed carbon (SPCE) electrodes focused on composite PEDOT:PSS/TiC/GQD for efficient detection using an enzyme-based biosensor.
基于酶的电化学生物传感器设备用于监测生化物质，仍被认为具有吸引力。我们研究了葡萄糖氧化酶（GOx）在新型复合纳米材料聚（3,4-亚乙基二氧噻吩）：聚苯乙烯磺酸盐（PEDOT：PSS）/碳化钛（TiC）/石墨烯量子点（GQD）修饰的丝网印刷碳电极（SPCE）上的固定化，用于葡萄糖传感。通过傅里叶变换红外光谱（FTIR）、X 射线光电子能谱（XPS）、扫描电子显微镜（SEM）、循环伏安法（CV）和差分脉冲伏安法（DPV）分析了 PEDOT：PSS/TiC/GQD 对 GOx 电催化氧化的特性和电化学行为。与单独的 PEDOT：PSS 和 PEDOT：PSS/TiC 相比，这种复合纳米材料更倾向于增加电化学行为，导致更高的峰值电流为 100.17 µA。此外，检测结果表明，所制备的生物传感器在 0-500 µM 的葡萄糖浓度范围内具有线性伏安响应，相对灵敏度为 21.64 µAmMcm，检测限为 65 µM（S/N = 3），具有良好的稳定性和选择性。这一发现可作为在基于酶的生物传感器中使用复合 PEDOT：PSS/TiC/GQD 修饰丝网印刷碳（SPCE）电极的有效检测的应用指导。
