McFadden Amj, Ha H J, Donald J J, Bueno I M, van Andel M, Thompson J C, Tisdall D J, Pulford D J
a Ministry for Primary Industries , 66 Ward Street, Wallaceville, Upper Hutt 5018 , New Zealand.
b New Zealand Veterinary Pathology , Cnr. Thackeray and Anglesea Streets, Hamilton 3206 , New Zealand.
N Z Vet J. 2016 Jan;64(1):65-8. doi: 10.1080/00480169.2015.1090356. Epub 2015 Oct 7.
A dairy cow, from a herd in the Waikato region of New Zealand, was reported with regenerative anaemia on 12 September 2014. Testing of blood from the animal using PCR assays for Theileria orientalis produced a negative result for both Chitose and Ikeda types.
Using PCR and DNA sequencing, blood from the cow was positive for Candidatus Mycoplasma haemobos. Further testing of another 12 animals from the case herd, 27 days after the affected cow was first reported, showed 11 animals were positive for Candidatus M. haemobos or Mycoplasma wenyonii in the PCR. None of these cattle were clinically anaemic or positive for T. orientalis Ikeda type using PCR. A convenience sample of 47 blood samples from cattle throughout New Zealand, submitted to the Investigation and Diagnostic Centre (Ministry for Primary Industries) for surveillance testing for T. orientalis Ikeda, was selected for further testing for bovine haemoplasmas. Of these samples, 6/47 (13%) and 13/47(28%) were positive for M. wenyonii and Candidatus M. haemobos, respectively. There was no difference in the proportion of samples positive for the bovine haemaplasmas between cattle with anaemia that were negative for T. orientalis (6/20, 33%), or without anaemia or T. orientalis (10/18, 56%), or from cattle herds experiencing anaemia and infection with T. orientalis Ikeda type (3/9, 33%).
Bovine haemoplasmosis.
The presence of bovine haemoplasmas in blood does not establish causality for anaemia in cattle. Diagnosis of anaemia associated with haemoplasmosis would require exclusion of other causes of regenerative anaemia and an association of the agent with anaemia in affected cattle herds. The data collected in this study did not provide evidence that bovine haemoplasmas were associated with a large number of outbreaks of anaemia in cattle in New Zealand.
2014年9月12日，一头来自新西兰怀卡托地区某牛群的奶牛被报告患有再生性贫血。使用针对东方泰勒虫的聚合酶链反应（PCR）检测该动物的血液，对千歳型和池田型均得出阴性结果。
通过PCR和DNA测序，该奶牛的血液对嗜血性隐秘支原体呈阳性。在首次报告患病奶牛27天后，对同病例牛群中的另外12头动物进行进一步检测，结果显示11头动物在PCR检测中对嗜血性隐秘支原体或温氏支原体呈阳性。这些牛均无临床贫血症状，且使用PCR检测对东方泰勒虫池田型呈阴性。从新西兰各地的牛群中选取了47份血液样本作为便利样本，提交给初级产业部调查与诊断中心进行东方泰勒虫池田型的监测检测，并对其进行牛血支原体的进一步检测。在这些样本中，分别有6/47（13%）和13/47（28%）对温氏支原体和嗜血性隐秘支原体呈阳性。对于东方泰勒虫呈阴性的贫血牛（6/20，33%）、无贫血症状或无东方泰勒虫的牛（10/18，56%）或经历贫血且感染东方泰勒虫池田型的牛群中的牛（3/9，33%），牛血支原体阳性样本的比例没有差异。
牛血支原体病。
血液中存在牛血支原体并不能确定其与牛贫血之间存在因果关系。与血支原体病相关的贫血诊断需要排除再生性贫血的其他病因，并确定病原体与受影响牛群贫血之间的关联。本研究收集的数据并未提供证据表明牛血支原体与新西兰大量牛贫血疫情有关。
