Animal Genome & Bioinformatics, National Institute of Animal Science, RDA, Wanju 55365, Korea.
Poultry Research Institute, National Institute of Animal Science, RDA, Pyeongchang 25342, Korea.
Genes (Basel). 2021 Jul 29;12(8):1170. doi: 10.3390/genes12081170.
Meat from Korean native chickens (KNCs) has high consumer demand; however, slow growth performance and high variation in body weight (BW) of KNCs remain an issue. Genome-wide association study (GWAS) is a powerful method to identify quantitative trait-associated genomic loci. A GWAS, based on a large-scale KNC population, is needed to identify underlying genetic mechanisms related to its growth traits. To identify BW-associated genomic regions, we performed a GWAS using the chicken 60K single nucleotide polymorphism (SNP) panel for 1328 KNCs. BW was measured at 8 weeks of age, from 2018 to 2020. Twelve SNPs were associated with BW at the suggestive significance level ( < 2.95 × 10) and located near or within 11 candidate genes, including , , , , and . Gene set enrichment analysis based on the GWAS results at < 0.05 (1680 SNPs) showed that 32 Gene Ontology terms and two Kyoto Encyclopedia of Genes and Genomes pathways, including regulation of transcription, motor activity, the mitogen-activated protein kinase signaling pathway, and tight junction, were significantly enriched ( < 0.05) for BW-associated genes. These pathways are involved in cell growth and development, related to BW gain. The identified SNPs are potential biomarkers in KNC breeding.
韩国土鸡（KNC）的肉质受到消费者的高度青睐；然而，其生长性能缓慢且体重波动较大，仍是 KNC 养殖所面临的问题。全基因组关联研究（GWAS）是一种识别与数量性状相关的基因组位点的强大方法。为了鉴定与生长性状相关的遗传机制，我们对一个大规模的 KNC 群体进行了 GWAS 分析，使用了鸡 60K 单核苷酸多态性（SNP）面板，共检测了 1328 只 KNC。体重在 2018 年至 2020 年的 8 周龄时进行测量。在提示性显著水平（ < 2.95×10）下，有 12 个 SNP 与 BW 相关，位于 11 个候选基因附近或之内，包括、、、、和。基于 GWAS 结果在 < 0.05（1680 个 SNP）的基因集富集分析表明，32 个基因本体论术语和两个京都基因与基因组百科全书途径，包括转录调控、运动活性、丝裂原激活蛋白激酶信号通路和紧密连接，与 BW 相关基因显著富集（ < 0.05）。这些途径涉及细胞生长和发育，与 BW 增加有关。鉴定出的 SNP 是 KNC 育种的潜在生物标志物。
