Li Fuwei, Han Haixia, Lei Qiuxia, Gao Jinbo, Liu Jie, Liu Wei, Zhou Yan, Li Huimin, Cao Dingguo
Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan, 250023, People's Republic of China.
Poultry Breeding Engineering Technology Center of Shandong Province, Jinan, 250023, Shandong, China.
J Appl Genet. 2018 Aug;59(3):305-312. doi: 10.1007/s13353-018-0452-7. Epub 2018 Jun 27.
Chicken body weight (BW) is an economically important trait, and many studies have been conducted on genetic selection for BW. However, previous studies have detected functional chromosome mutations or regions using gene chips. The present study used the specific-locus amplified fragment sequencing (SLAF-seq) technology to perform a genome-wide association study (GWAS) on purebred Wengshang Barred chicken. A total of 1,286,715 single-nucleotide polymorphisms (SNPs) were detected, and 175,211 SNPs were selected as candidate SNPs for genome-wide association analysis using TASSEL general linear models. Six SNP markers reached genome-wide significance. Of these, rs732048524, rs735522839, rs738991545, and rs15837818 were significantly associated with body weight at 28 days (BW28), while rs314086457 and rs315694878 were significantly associated with BW120. These SNPs are close to seven genes (PRSS23, ME3, FAM181B, NABP1, SDPR, TSSK6L2, and RBBP8). Moreover, 24 BW-associated SNPs reached "suggestive" genome-wide significance. Of these, 6, 13, 1, and 4 SNPs were associated with BW28, BW56, BW80, and BW120, respectively. These results would enrich the studies on BW and promote the use of Chinese chicken, especially the Wenshang Barred chicken.
鸡的体重是一个具有重要经济意义的性状，并且已经针对体重的遗传选择开展了许多研究。然而，以往的研究是使用基因芯片检测功能染色体突变或区域。本研究采用特定位点扩增片段测序（SLAF-seq）技术，对纯种汶上芦花鸡进行全基因组关联研究（GWAS）。共检测到1,286,715个单核苷酸多态性（SNP），并使用TASSEL一般线性模型选择了175,211个SNP作为全基因组关联分析的候选SNP。六个SNP标记达到全基因组显著性水平。其中，rs732048524、rs735522839、rs738991545和rs15837818与28日龄体重（BW28）显著相关，而rs314086457和rs315694878与120日龄体重（BW120）显著相关。这些SNP靠近七个基因（PRSS23、ME3、FAM181B、NABP1、SDPR、TSSK6L2和RBBP8）。此外，24个与体重相关的SNP达到“提示性”全基因组显著性水平。其中，分别有6个、13个、1个和4个SNP与BW28、BW56、BW80和BW120相关。这些结果将丰富对体重的研究，并促进中国鸡尤其是汶上芦花鸡的利用。
