Department of Food Engineering and Process Management, Institute of Food Sciences, Warsaw University of Life Sciences, 159c Nowoursynowska St., 02-776 Warsaw, Poland.
Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, 159c Nowoursynowska St., 02-776 Warsaw, Poland.
Molecules. 2021 Aug 6;26(16):4773. doi: 10.3390/molecules26164773.
The polyphenol content of tea depends on the growing region, harvest date, the production process used, and the brewing parameters. In this study, research was undertaken that included an analysis of the influence of the brewing process parameters on the content of total polyphenols (Folin-Ciocalteu), epigallocatechin gallate (HPLC), and antioxidant activity (against DPPH radicals) of fresh tea shrub leaves grown from Taiwan and of teas obtained from them (oolong, green in bags, and green loose from the spring and autumn harvest). The antioxidant potential was determined in the methanol and aqueous extracts, as well as in infusions that were obtained by using water at 65 or 100 °C and infusing the tea for 5 or 10 min. The highest content of total polyphenols and epigallocatechin gallate was found in green tea extracts from the spring harvest. However, in the case of infusions, the highest content of these compounds was found in green tea in bags. Steaming at 100 °C for 10 min, turned out to be the most favourable condition for the extraction. Oolong tea, brewed at 100 °C for 5 min was characterised by the highest antioxidant activity against stable DPPH radicals.
茶叶中的多酚含量取决于生长地区、收获日期、使用的生产工艺以及冲泡参数。本研究分析了冲泡工艺参数对来自台湾的新鲜茶树叶片及其制成的茶(乌龙茶、袋泡茶绿茶和春秋两季采摘的散装绿茶)中总多酚(福林-考克法)、表没食子儿茶素没食子酸酯(HPLC)含量和抗氧化活性(对 DPPH 自由基)的影响。在甲醇和水提取物中以及在 65 或 100°C 水提取并分别浸泡 5 或 10 分钟获得的浸液中,测定了抗氧化能力。春茶绿茶提取物中总多酚和表没食子儿茶素没食子酸酯含量最高。然而,就浸液而言,袋泡茶绿茶的这些化合物含量最高。100°C 蒸汽蒸 10 分钟被证明是最有利于提取的条件。100°C 冲泡 5 分钟的乌龙茶具有最高的稳定 DPPH 自由基清除抗氧化活性。