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人源脱细胞同种异体骨骨髓间充质干细胞、脂肪组织和牙髓间充质干细胞成骨和成软骨特性分析。

Characterization of Osteogenesis and Chondrogenesis of Human Decellularized Allogeneic Bone with Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Wharton's Jelly.

机构信息

Department of Orthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei 11217, Taiwan.

Department of Orthopaedics, School of Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan.

出版信息

Int J Mol Sci. 2021 Aug 20;22(16):8987. doi: 10.3390/ijms22168987.

DOI:10.3390/ijms22168987
PMID:34445692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8396436/
Abstract

Allogeneic bone grafts are a promising material for bone implantation due to reduced operative trauma, reduced blood loss, and no donor-site morbidity. Although human decellularized allogeneic bone (hDCB) can be used to fill bone defects, the research of revitalizing hDCB blocks with human mesenchymal stem cells (hMSCs) for osteochondral regeneration is missing. The hMSCs derived from bone marrow, adipose tissue, and Wharton's jelly (BMMSCs, ADMSCs, and UMSCs, respectively) are potential candidates for bone regeneration. This study characterized the potential of hDCB as a scaffold for osteogenesis and chondrogenesis of BMMSCs, ADMSCs, and UMSCs. The pore sizes and mechanical strength of hDCB were characterized. Cell survival and adhesion of hMSCs were investigated using MTT assay and F-actin staining. Alizarin Red S and Safranin O staining were conducted to demonstrate calcium deposition and proteoglycan production of hMSCs after osteogenic and chondrogenic differentiation, respectively. A RT-qPCR was performed to analyze the expression levels of osteogenic and chondrogenic markers in hMSCs. Results indicated that BMMSCs and ADMSCs exhibited higher osteogenic potential than UMSCs. Furthermore, ADMSCs and UMSCs had higher chondrogenic potential than BMMSCs. This study demonstrated that chondrogenic ADMSCs- or UMSCs-seeded hDCB might be potential osteochondral constructs for osteochondral regeneration.

摘要

同种异体骨移植物因其手术创伤小、失血量少、供区无发病率而成为骨植入的有前途的材料。虽然脱细胞异体人骨 (hDCB) 可用于填充骨缺损，但用人间充质干细胞 (hMSCs) 使 hDCB 块复活以进行骨软骨再生的研究却缺失了。来源于骨髓、脂肪组织和沃顿胶（分别为 BMMSCs、ADMSCs 和 UMSCs）的 hMSCs 是骨再生的潜在候选者。本研究表征了 hDCB 作为 BMMSCs、ADMSCs 和 UMSCs 成骨和成软骨的支架的潜力。对 hDCB 的孔径和机械强度进行了表征。通过 MTT 测定法和 F-肌动蛋白染色研究了 hMSC 的细胞存活和黏附。茜素红 S 和番红 O 染色分别用于证明 hMSCs 在成骨和软骨分化后钙沉积和蛋白聚糖的产生。进行了 RT-qPCR 以分析 hMSC 中成骨和软骨标志物的表达水平。结果表明，BMMSCs 和 ADMSCs 比 UMSCs 具有更高的成骨潜力。此外，ADMSCs 和 UMSCs 比 BMMSCs 具有更高的成软骨潜力。本研究表明，ADMSCs 或 UMSCs 种子化的 hDCB 可能是用于骨软骨再生的潜在的骨软骨构建体。

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