Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran Iran.
Acta Trop. 2021 Dec;224:106109. doi: 10.1016/j.actatropica.2021.106109. Epub 2021 Aug 24.
A total of 90 stool samples were collected from dogs, referred to a dog shelter and a veterinary clinic. In addition, 395 stool samples obtained from pet dog owners and shelter keepers, as well as individuals referred to a medical laboratory as controls, were collected in Shahryar district, Tehran, Iran. Stool samples were parasitologically examined and the positive G. lamblia isolates were tested with Nested-PCR/sequencing for the tpi, gdh, and bg genes, and HRM real-time PCR. Microscopical examination revealed 20 (22.2%) and 34 (8.6%) Giardia-positive samples from dogs and humans, respectively. Regarding HRM real-time PCR, the prevalence of assemblages A and B in humans was 55.8% and 14.7%, respectively. In addition, 14.7% of samples were mix assemblages. HRM real-time PCR detected most of microscopically-positive samples in comparison to PCR/sequencing in both humans and dogs. The high prevalence of assemblages A and B in dogs signified the importance of a same source for infection between dogs and humans.
共采集 90 份来自狗的粪便样本，这些样本分别来自一家狗收容所和一家兽医诊所。此外，在伊朗德黑兰沙阿里亚尔区，还采集了 395 份来自宠物狗主人和收容所饲养员以及作为对照被送往医学实验室的个体的粪便样本。对粪便样本进行寄生虫学检查，对阳性的蓝氏贾第鞭毛虫分离株用巢式 PCR/测序检测 tpi、gdh 和 bg 基因，并进行 HRM 实时 PCR。显微镜检查显示，狗和人类的贾第鞭毛虫阳性样本分别为 20（22.2%）和 34（8.6%）。关于 HRM 实时 PCR，人类中 A 和 B 组的流行率分别为 55.8%和 14.7%。此外，14.7%的样本为混合组。与 PCR/测序相比，HRM 实时 PCR 在人类和狗中均检测到了大多数显微镜阳性样本。狗中 A 组和 B 组的高流行率表明了狗和人类之间感染源相同的重要性。
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