伊朗东部南呼罗珊省贾第虫的 HRM 实时 PCR 方法的集合特征分析。
Assemblage characterization of Giardia duodenalis in South Khorasan province, eastern Iran, using HRM real-time PCR method.
机构信息
Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran.
出版信息
Mol Biol Rep. 2024 Jan 18;51(1):127. doi: 10.1007/s11033-023-09001-3.
BACKGROUND
Giardia duodenalis is a common parasitic protozoan causing gastrointestinal illness in humans worldwide. The genetic diversity of G. duodenalis is reflected through the identification of different assemblages. In this study, we aimed to determine the assemblages of G. duodenalis in eastern Iran using nested-PCR and high-resolution melting (HRM) real-time PCR methods.
METHODS
A total of 58 positive G. duodenalis, which were isolated from 1800 subjects, referred to medical center laboratories in South Khorasan province, eastern Iran, from April 2020 to March 2022, were included in this study. DNA was extracted and HRM real-time PCR was performed for assemblage characterization.
RESULTS
HRM real-time PCR successfully characterized all samples. Accordingly, out of 58 positive samples, 53 (91.36%) and 5 (8.62%) were identified as assemblage A and B, respectively.
CONCLUSIONS
Our findings showed that HRM real-time PCR was able to characterize the assemblages of G. duodenalis. In addition, our results suggest high prevalence of assemblage A in eastern region of Iran.
背景
十二指肠贾第鞭毛虫是一种常见的寄生原虫，在全球范围内引起人类胃肠道疾病。通过鉴定不同的集合体，可以反映出十二指肠贾第鞭毛虫的遗传多样性。本研究旨在采用巢式 PCR 和高分辨率熔解（HRM）实时 PCR 方法，确定伊朗东部地区的 G. duodenalis 集合体。
方法
从 2020 年 4 月至 2022 年 3 月，共收集了来自伊朗南部科拉桑省医疗中心实验室的 1800 名患者中分离出的 58 株阳性 G. duodenalis，对其进行了 HRM 实时 PCR 分析以鉴定集合体特征。
结果
HRM 实时 PCR 成功地对所有样本进行了鉴定。因此，在 58 个阳性样本中，53 个（91.36%）和 5 个（8.62%）被鉴定为 A 集合体和 B 集合体。
结论
本研究表明 HRM 实时 PCR 能够鉴定 G. duodenalis 的集合体。此外，本研究结果表明，伊朗东部地区 A 集合体的流行率较高。
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