Institute of Robotics and Automatic Information System, Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China.
Institute of Animal Sciences, Tianjin 300112, China.
Sensors (Basel). 2021 Aug 18;21(16):5561. doi: 10.3390/s21165561.
The mechanical properties of biological cells, especially the elastic modulus and viscosity of cells, have been identified to reflect cell viability and cell states. The existing measuring techniques need additional equipment or operation condition. This paper presents a cell's viscoelasticity measurement method based on the spheroidization process of non-spherical shaped cell. The viscoelasticity of porcine fetal fibroblast was measured. Firstly, we introduced the process of recording the spheroidization process of porcine fetal fibroblast. Secondly, we built the viscoelastic model for simulating a cell's spheroidization process. Then, we simulated the spheroidization process of porcine fetal fibroblast and got the simulated spheroidization process. By identifying the parameters in the viscoelastic model, we got the elasticity (500 Pa) and viscosity (10 Pa·s) of porcine fetal fibroblast. The results showed that the magnitude of the elasticity and viscosity were in agreement with those measured by traditional method. To verify the accuracy of the proposed method, we imitated the spheroidization process with silicone oil, a kind of viscous and uniform liquid with determined viscosity. We did the silicone oil's spheroidization experiment and simulated this process. The simulation results also fitted the experimental results well.
生物细胞的力学特性，尤其是细胞的弹性模量和黏度，已被确定为反映细胞活力和细胞状态的指标。现有的测量技术需要额外的设备或操作条件。本文提出了一种基于非球形细胞的球化过程的细胞黏弹性测量方法。测量了猪胎儿成纤维细胞的黏弹性。首先，我们介绍了记录猪胎儿成纤维细胞球化过程的过程。其次，我们建立了模拟细胞球化过程的黏弹性模型。然后，我们模拟了猪胎儿成纤维细胞的球化过程，并得到了模拟的球化过程。通过识别黏弹性模型中的参数，我们得到了猪胎儿成纤维细胞的弹性（500 Pa）和黏性（10 Pa·s）。结果表明，弹性和黏性的大小与传统方法测量的结果一致。为了验证所提出方法的准确性，我们用硅油模拟了球化过程，硅油是一种具有确定黏度的粘性和均匀的液体。我们进行了硅油的球化实验并模拟了这个过程。模拟结果也很好地拟合了实验结果。
