Lukas Brigitte, Jovanovic Dijana, Schmiderer Corinna, Kostas Stefanos, Kanellis Angelos, Gómez Navarro José, Aytaç Zehra, Koç Ali, Sözen Emel, Novak Johannes
Institute of Animal Nutrition and Functional Plant Compounds, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
Department of Horticulture, School of Agriculture, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.
Plants (Basel). 2021 Aug 6;10(8):1619. doi: 10.3390/plants10081619.
(Cistaceae) comprises a number of white- and purple-flowering shrub species widely distributed in the Mediterranean basin. Within genus many taxa are subject to various taxonomic uncertainties. , a prominent member of the purple-flowered clade, is a prime case of the current taxonomic troubles. Floras and databases approve different species names and utilise different or additional/fewer synonyms. Various intraspecific classification systems based on subspecies or varieties are in use. The inconsistent determination of plant material makes it difficult to compare literature regarding the phytochemical diversity and biological activities of plant material and impedes a systematic utilization of the manifold medicinal properties of . In the present investigation, we used DNA sequence data from one nuclear region (ITS) and two chloroplast regions (, ) to test the intraspecific genetic diversity of and its evolutionary relationships to the closely related . The combined DNA data confirmed as a rather heterogeneous species that integrates two major evolutionary lineages with clearly different genetic characteristics. The 'Eastern Mediterranean clade' seems to represent old and ancestral characteristics. This lineage exhibits a close relationship to the geographically distant expressed by very closely related ribotypes and an interspecifically shared chlorotype. The 'Western Mediterranean clade' is characterized by a distinctive ITS polymorphism (co-occurring paralogous ribotypes) and more distantly related chlorotypes. The formation of the genetically complex 'Western Mediterranean clade' seems to have involved hybridization and recurrent formation or migration movements.
半日花科包含多种开白花和紫花的灌木物种，广泛分布于地中海盆地。在该属内，许多分类单元存在各种分类学上的不确定性。作为紫花分支的一个突出成员，是当前分类学难题的一个典型例子。植物志和数据库认可不同的物种名称，并使用不同的或额外的/更少的同义词。基于亚种或变种的各种种内分类系统正在使用。植物材料鉴定的不一致使得难以比较关于植物材料的植物化学多样性和生物活性的文献，并且阻碍了对其多种药用特性的系统利用。在本研究中，我们使用来自一个核区域（ITS）和两个叶绿体区域（，）的DNA序列数据来测试的种内遗传多样性及其与近缘的进化关系。合并的DNA数据证实是一个相当异质的物种，整合了两个具有明显不同遗传特征的主要进化谱系。“东地中海分支”似乎代表古老和祖先特征。该谱系与地理上遥远的表现出密切关系，通过非常密切相关的核糖体型和种间共享的叶绿体型来体现。“西地中海分支”的特征是独特的ITS多态性（同时出现的旁系核糖体型）和关系较远的叶绿体型。遗传复杂的“西地中海分支”的形成似乎涉及杂交和反复形成或迁移运动。
