Smeriglio Antonella, Denaro Marcella, Trombetta Domenico, Ragusa Salvatore, Circosta Clara
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno d'Alcontres 31, 98166 Messina, Italy.
Department of Health Sciences, University "Magna Graecia" of Catanzaro, Viale Europa, 88100 Catanzaro, Italy.
Plants (Basel). 2021 Aug 6;10(8):1621. doi: 10.3390/plants10081621.
L. is a rounded shrub commonly found in the Mediterranean area well-known, since ancient times, for its traditional use. The aim of the present study was to investigate the phytochemical profile as well as the antioxidant and anti-inflammatory properties of flower (FE), leaf (LE), fruit (FrE), and branch (BE) hydroalcoholic extracts. For this purpose, a preliminary phytochemical screening followed by RP-LC-DAD-ESI-MS analysis, as well as several in vitro cell-free colorimetric assays, were carried out. Moreover, the toxicity of the extracts was investigated by the brine shrimp lethality assay. All extracts showed a high content of polyphenols, in particular phenolic acids (chlorogenic acid 0.74-13.80 g/100 g) and flavonoids (rutin 0.05-2.76 g/100 g and isovitexin 8.02 in BE). All the extracts showed strong and concentration-dependent antioxidant and anti-inflammatory activity with, on average, the following order of potency: FE, LE, FrE, and BE. Interestingly, all the extracts investigated did not show any toxicity on . Moreover, the only LD found (BE, 8.82 mg/mL) is well above the concentration range, which has been shown the biological properties. Considering this, this study offers the first evidence of the possible use of the polyphenol extracts from the aerial parts of as promising antioxidant and anti-inflammatory agents.
L. 是一种常见于地中海地区的圆形灌木，自古以来就因其传统用途而闻名。本研究的目的是调查花（FE）、叶（LE）、果实（FrE）和枝条（BE）水醇提取物的植物化学特征以及抗氧化和抗炎特性。为此，进行了初步的植物化学筛选，随后进行了RP-LC-DAD-ESI-MS分析以及几种体外无细胞比色测定。此外，通过卤虫致死试验研究了提取物的毒性。所有提取物均显示出高含量的多酚，特别是酚酸（绿原酸0.74 - 13.80 g/100 g）和黄酮类化合物（芦丁0.05 - 2.76 g/100 g，BE中的异荭草素8.02）。所有提取物均表现出强烈的且浓度依赖性的抗氧化和抗炎活性，平均效力顺序如下：FE、LE、FrE和BE。有趣的是，所有研究的提取物对……均未显示出任何毒性。此外，唯一发现的LD（BE，8.82 mg/mL）远高于已显示出生物学特性的浓度范围。考虑到这一点，本研究首次证明了从……地上部分提取的多酚提取物作为有前景的抗氧化和抗炎剂的可能用途。
