Zhang Kaijing, Jia Li, Yang Dekun, Hu Yuchao, Njogu Martin Kagiki, Wang Panqiao, Lu Xiaomin, Yan Congsheng
College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China.
Key Laboratory of Genetic Improvement and Ecophysiology of Horticultural Crop, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230001, China.
Plants (Basel). 2021 Aug 7;10(8):1626. doi: 10.3390/plants10081626.
GATA transcription factors are a class of transcriptional regulatory proteins that contain a characteristic type-IV zinc finger DNA-binding domain, which play important roles in plant growth and development. The GATA gene family has been characterized in various plant species. However, GATA family genes have not been identified in cucumber. In this study, 26 GATA family genes were identified in cucumber genome, whose physicochemical characteristics, chromosomal distributions, phylogenetic tree, gene structures conserved motifs, -regulatory elements in promoters, homologous gene pairs, downstream target genes were analyzed. Tissue expression profiles of cucumber GATA family genes exhibited that 17 GATA genes showed constitutive expression, and some GATA genes showed tissue-specific expression patterns. RNA-seq analysis of green and virescent leaves revealed that seven GATA genes might be involved in the chloroplast development and chlorophyll biosynthesis. Importantly, expression patterns analysis of GATA genes in response to abiotic and biotic stresses indicated that some GATA genes respond to either abiotic stress or biotic stress, some GATA genes such as Csa2G162660, Csa3G017200, Csa3G165640, Csa4G646060, Csa5G622830 and Csa6G312540 were simultaneously functional in resistance to abiotic and biotic stresses. Overall, this study will provide useful information for further analysis of the biological functions of GATA factors in cucumber.
GATA转录因子是一类转录调节蛋白，其包含一个特征性的IV型锌指DNA结合结构域，在植物生长发育中发挥重要作用。GATA基因家族已在多种植物物种中得到鉴定。然而，黄瓜中尚未鉴定出GATA家族基因。在本研究中，在黄瓜基因组中鉴定出26个GATA家族基因，并对其理化特性、染色体分布、系统发育树、基因结构、保守基序、启动子中的调控元件、同源基因对、下游靶基因进行了分析。黄瓜GATA家族基因的组织表达谱显示，17个GATA基因呈组成型表达，一些GATA基因表现出组织特异性表达模式。对绿色和浅绿色叶片的RNA测序分析表明，7个GATA基因可能参与叶绿体发育和叶绿素生物合成。重要的是，GATA基因对非生物和生物胁迫响应模式的分析表明，一些GATA基因对非生物胁迫或生物胁迫有响应，一些GATA基因如Csa2G162660、Csa3G017200、Csa3G165640、Csa4G646060、Csa5G622830和Csa6G312540在抗非生物和生物胁迫中同时发挥作用。总体而言，本研究将为进一步分析黄瓜中GATA因子的生物学功能提供有用信息。
