Zhao Fangfang, Li Xia, Chen Ziqi, Guo Changhong
Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, China.
Jilin Provincial Key Laboratory of Agricultural Biotechnology, Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences (Northeast Innovation Center of Agricultural Science and Technology in China), Changchun 130124, China.
Plants (Basel). 2025 Jun 1;14(11):1693. doi: 10.3390/plants14111693.
GATA is a crucial transcription factor involved in plant growth, development, and responses to abiotic stress. Therefore, identifying and exploring GATA transcription factors in maize is of significant importance. In this study, we identified 75 genes based on the pan-genome of maize, which includes 26 high-quality maize genomes. These consist of 58 core genes (present in all 26 lines), 12 non-essential genes (present in 2 to 23 lines), 2 near-core genes (present in 24 to 25 lines), and 3 private genes (present in only 1 line). By evaluating the Ka/Ks ratio of the genes in 26 maize varieties, we found that the Ka/Ks ratios of , , , and were greater than 1, which may indicate that these four genes are under positive selection. In contrast, the Ka/Ks ratios of other genes were less than 1, suggesting that these genes may be under purifying selection. In the 26 maize genomes, we observed a significant difference in the expression of between varieties affected by structural variations (SVs) and those not affected. In certain varieties, SVs altered conserved structures. Additionally, we analyzed the expression levels of genes in different maize tissues and under abiotic stress. and were highly expressed in the endosperm, thereby influencing starch synthesis, while , , , , and were found to be associated with abiotic stress responses. These findings provide valuable new resources for functional research on .
GATA是一种关键的转录因子，参与植物的生长、发育以及对非生物胁迫的响应。因此，鉴定和探索玉米中的GATA转录因子具有重要意义。在本研究中，我们基于玉米的泛基因组鉴定了75个基因，该泛基因组包括26个高质量的玉米基因组。这些基因由58个核心基因（存在于所有26个品系中）、12个非必需基因（存在于2至23个品系中）、2个近核心基因（存在于24至25个品系中）和3个私有基因（仅存在于1个品系中）组成。通过评估26个玉米品种中这些基因的Ka/Ks比值，我们发现[具体基因名称1]、[具体基因名称2]、[具体基因名称3]和[具体基因名称4]的Ka/Ks比值大于1，这可能表明这四个基因正处于正选择之下。相比之下，其他基因的Ka/Ks比值小于1，表明这些基因可能处于纯化选择之下。在26个玉米基因组中，我们观察到受结构变异（SVs）影响的品种与未受影响的品种之间[具体基因名称]的表达存在显著差异。在某些品种中，SVs改变了保守结构。此外，我们分析了[具体基因名称]在不同玉米组织和非生物胁迫下的表达水平。[具体基因名称5]和[具体基因名称6]在胚乳中高度表达，从而影响淀粉合成，而[具体基因名称7]、[具体基因名称8]、[具体基因名称9]、[具体基因名称10]和[具体基因名称11]被发现与非生物胁迫响应相关。这些发现为[具体基因名称]的功能研究提供了有价值的新资源。
