Kim Do Kyung, Rhee Won Jong
Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon 22012, Korea.
Division of Bioengineering, Incheon National University, Incheon 22012, Korea.
Pharmaceutics. 2021 Aug 5;13(8):1203. doi: 10.3390/pharmaceutics13081203.
Oxidative stress is implicated in many diseases, including cardiovascular and neurodegenerative diseases. Because an increased level of oxidative stress causes apoptosis, it is necessary to inhibit cellular responses to oxidative stress. In this study, Carex, a nanovesicle from carrot, was isolated and investigated as a novel biomaterial with antioxidative function in cardiomyoblasts and neuroblastoma cells. A high concentration of nanovesicles was purified from carrots, using size-exclusion chromatography in combination with ultrafiltration. The characterization of Carex demonstrated that it had properties similar to those of extracellular vesicles. Carex showed low cytotoxicity in both H9C2 cardiomyoblasts and SH-SY5Y neuroblastoma cells, when a high level of Carex was delivered to the cells. Carex was further investigated for its antioxidative and apoptotic effects, and it significantly inhibited ROS generation and apoptosis in vitro in myocardial infarction and Parkinson's disease models. Carex inhibited the reduction of antioxidative molecule expression, including Nrf-2, HO-1, and NQO-1, in both models. Considering its antioxidative function and high production yield, Carex is a potential drug candidate for the treatment of myocardial infarction as well as Parkinson's disease. Thus, the results demonstrated in this study will contribute to an exploration of a novel drug, using nanovesicles from plants, including carrots.
氧化应激与包括心血管疾病和神经退行性疾病在内的多种疾病有关。由于氧化应激水平的升高会导致细胞凋亡，因此有必要抑制细胞对氧化应激的反应。在本研究中，从胡萝卜中分离出一种名为Carex的纳米囊泡，并将其作为一种在心肌母细胞和神经母细胞瘤细胞中具有抗氧化功能的新型生物材料进行研究。通过尺寸排阻色谱结合超滤从胡萝卜中纯化出高浓度的纳米囊泡。对Carex的表征表明，它具有与细胞外囊泡相似的特性。当向H9C2心肌母细胞和SH-SY5Y神经母细胞瘤细胞递送高水平的Carex时，Carex在这两种细胞中均表现出低细胞毒性。进一步研究了Carex的抗氧化和凋亡作用，结果表明它在心肌梗死和帕金森病模型中能显著抑制体外活性氧的生成和细胞凋亡。在这两种模型中，Carex均抑制了包括Nrf-2、HO-1和NQO-1在内的抗氧化分子表达的降低。考虑到其抗氧化功能和高产率，Carex是治疗心肌梗死和帕金森病的潜在候选药物。因此，本研究结果将有助于探索使用包括胡萝卜在内的植物纳米囊泡开发新型药物。
