Dabasinskaite Lauryna, Krugly Edvinas, Baniukaitiene Odeta, Martuzevicius Dainius, Ciuzas Darius, Jankauskaite Lina, Aukstikalne Lauryna, Usas Arvydas
Department of Environmental Technology, Kaunas University of Technology, LT-50254 Kaunas, Lithuania.
Department of Polymer Chemistry and Technology, Kaunas University of Technology, LT-50254 Kaunas, Lithuania.
Pharmaceutics. 2021 Aug 18;13(8):1288. doi: 10.3390/pharmaceutics13081288.
Ozonation has been proved as a viable surface modification technique providing certain properties to the scaffolds that are essential in tissue engineering. However, the ozone (O) treatment of PCL scaffolds in aqueous environments has not yet been presented. O treatment performed in aqueous environments is more effective compared with traditional, executed in ambient air treatment due to more abundant production of hydroxyl radicals (•OH) within the O reaction with water molecules. During interaction with •OH, the scaffold acquires functional groups which improve wettability properties and encapsulate growth factors. In this study, a poly(ε)caprolactone (PCL) scaffold was fabricated using solution electrospinning and was subsequently ozonated in a water reactor. The O treatment resulted in the expected occurrence of oxygen-containing functional groups, which improved scaffold wettability by almost 27% and enhanced cell proliferation for up to 14 days. The PCL scaffold was able to withhold 120 min of O treatment, maintaining fibrous morphology and mechanical properties.
臭氧化已被证明是一种可行的表面改性技术，可为组织工程中至关重要的支架提供某些特性。然而，尚未有关于在水环境中对聚己内酯（PCL）支架进行臭氧（O）处理的报道。与在环境空气中进行的传统处理相比，在水环境中进行的O处理更为有效，这是因为在O与水分子的反应中会产生更丰富的羟基自由基（•OH）。在与•OH相互作用的过程中，支架获得了改善润湿性并封装生长因子的官能团。在本研究中，使用溶液静电纺丝制备了聚（ε）己内酯（PCL）支架，随后在水反应器中对其进行臭氧化处理。O处理导致预期的含氧基官能团出现，使支架润湿性提高了近27%，并在长达14天的时间内增强了细胞增殖。PCL支架能够承受120分钟的O处理，同时保持纤维形态和机械性能。
