Affiliated Eye Hospital, Wenzhou Medical College, Wenzhou, Zhejiang, People's Republic of China.
J Mater Sci Mater Med. 2012 Feb;23(2):547-54. doi: 10.1007/s10856-011-4495-0. Epub 2011 Dec 6.
Development of fibrous scaffold of hydroxyapatite/biopolymer nanocomposite offers great potential in the field of bone regeneration and tissue engineering. Hydroxyapatite (HA)/poly (ε-caprolactone) (PCL) fibrous scaffolds were successfully prepared by electrospinning dopes containing HA and PCL in this work. It was found that pre-treating HA with γ-glycioxypropyltrimethoxysilane (A-187) was effective in improving HA dispersion both in solutions and in a PCL matrix. Mechanical properties of the scaffolds were greatly enhanced by the filling of A187-HA. The bioactivity of PCL was remarkably improved by the addition of HA and A187-HA. Fibroblasts and osteoblasts were seeded on scaffolds to evaluate the effect of A-187 on biocompatibility of HA/PCL composites. Based on this study, good dispersion of HA in PCL matrix was granted by pretreatment of HA with A-187 and A187-HA/PCL fibrous scaffolds were obtained by electrospinning. These results demonstrated that the scaffolds may possess improved mechanical performance and good bioactivity due to A187-HA incorporation.
羟基磷灰石/生物聚合物纳米复合材料纤维支架的开发在骨再生和组织工程领域具有巨大的潜力。本工作通过静电纺丝法成功制备了含有羟基磷灰石（HA）和聚（ε-己内酯）（PCL）的纺丝液，制备了 HA/PCL 纤维支架。研究发现，用γ-缩水甘油丙基三甲氧基硅烷（A-187）对 HA 进行预处理，可有效改善 HA 在溶液和 PCL 基体中的分散性。A187-HA 的填充使支架的力学性能大大提高。HA 和 A187-HA 的加入显著提高了 PCL 的生物活性。将成纤维细胞和成骨细胞接种到支架上，以评估 A-187 对 HA/PCL 复合材料生物相容性的影响。基于这项研究，通过用 A-187 对 HA 进行预处理，HA 在 PCL 基体中得到了很好的分散，并通过静电纺丝得到了 A187-HA/PCL 纤维支架。这些结果表明，由于 A187-HA 的加入，支架可能具有改善的机械性能和良好的生物活性。
