Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, v.v.i, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
Viruses. 2021 Jul 29;13(8):1487. doi: 10.3390/v13081487.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the coronavirus disease-19 pandemic. One of the key components of the coronavirus replication complex are the RNA methyltransferases (MTases), RNA-modifying enzymes crucial for RNA cap formation. Recently, the structure of the 2'- MTase has become available; however, its biological characterization within the infected cells remains largely elusive. Here, we report a novel monoclonal antibody directed against the SARS-CoV-2 non-structural protein nsp10, a subunit of both the 2'- RNA and N7 MTase protein complexes. Using this antibody, we investigated the subcellular localization of the SARS-CoV-2 MTases in cells infected with the SARS-CoV-2.
严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）是导致 2019 冠状病毒病大流行的病原体。冠状病毒复制复合物的关键组成部分之一是 RNA 甲基转移酶（MTases），这是 RNA 帽形成所必需的 RNA 修饰酶。最近，2'- MTase 的结构已经可用；然而，其在感染细胞内的生物学特性在很大程度上仍难以捉摸。在这里，我们报告了一种针对 SARS-CoV-2 非结构蛋白 nsp10 的新型单克隆抗体，nsp10 是 2'- RNA 和 N7 MTase 蛋白复合物的一个亚单位。使用这种抗体，我们研究了 SARS-CoV-2 感染细胞中 SARS-CoV-2 MTases 的亚细胞定位。
