Mapother M E, Joens L A, Glock R D
University of Arizona, Veterinary Science Department, Tucson 85721.
Vet Rec. 1987 Dec 5;121(23):533-6.
Conventional crossbred pigs from different sources and of different weights were examined for susceptibility to porcine proliferative enteritis. The ileal mucosa of pigs with the disease was emulsified and suspended in Mueller-Hinton broth. Pigs weighing 15, 120 and 200 lb (6.8, 54.5 and 91 kg) (four pigs per group) were stressed and inoculated orally with 80 ml of emulsified proliferative ilea. Severe lesions of porcine proliferative enteritis were detected in three of the four pigs weighing 6.8 kg. Mild lesions were detected in two of the four pigs in each of the other two groups. Gross lesions consisted of reticulation of the serosa, and hyperaemia and thickening of the mucosa with either fibrin or blood clots adherent to the mucosal surface. Inflammation, numerous mitotic figures and epithelial cell proliferation were observed microscopically in the crypts. Silver stained sections revealed numerous comma-shaped organisms in the crypts of infected epithelial cells. Using this method, serial reproduction of the disease was accomplished through the passage of fresh and previously frozen inocula. The virulence of the freshly prepared inoculum increased with passage through the host, whereas the inoculum prepared from tissue that had previously been frozen showed a decrease in infectivity and virulence. These data provide strong evidence for the infectious nature of this disease.
对来自不同来源、不同体重的常规杂交猪进行了猪增生性肠炎易感性检测。将患病猪的回肠黏膜乳化并悬浮于Mueller-Hinton肉汤中。对体重分别为15磅、120磅和200磅（6.8千克、54.5千克和91千克）的猪（每组4头）进行应激处理，并经口接种80毫升乳化的增生性回肠炎组织。在体重6.8千克的4头猪中，有3头检测到严重的猪增生性肠炎病变。另外两组中，每组4头猪中有2头检测到轻度病变。大体病变包括浆膜呈网状、黏膜充血和增厚，黏膜表面附着有纤维蛋白或血凝块。显微镜下观察到隐窝中有炎症、大量有丝分裂象和上皮细胞增生。银染切片显示感染上皮细胞的隐窝中有大量逗号状微生物。通过这种方法，通过新鲜接种物和先前冷冻的接种物传代实现了该疾病的连续复制。新鲜制备的接种物在通过宿主传代后毒力增加，而由先前冷冻的组织制备的接种物则显示出感染性和毒力下降。这些数据为该疾病的传染性提供了有力证据。
