Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
National Research Council, Institute of Sciences of Food Production, Bari, Italy.
Microbiol Res. 2021 Nov;252:126855. doi: 10.1016/j.micres.2021.126855. Epub 2021 Aug 24.
Sugarcane is an important crop in Southern Iran for agri-food, energy, and pharmaceutical industries. Among the pathogens that colonize sugarcane, mycotoxigenic Fusarium species are reason of serious concern for both their pathogenicity on plants and ability to produce harmful mycotoxins to humans and animals. We studied 104 Fusarium strains, selected within a wider Fusarium set isolated from sugarcane in Southern Iran, for molecular identification, phylogeny and mycotoxin analyses. Most of Fusarium strains belonged to Fusarium fujikuroi Species Complex (FFSC) and identified mainly as F. proliferatum, at minor extent as F. sacchari, and rarely as F. thapsinum, and F. verticillioides. Moreover, 14 strains identified as FFSC could not be assigned to any known species, although they were phylogenetically closely related to F. andiyazi, likely representing a new phylogenetic species. A subset of FFSC strains were analyzed for in vitro production of fumonisins (FBs), beauvericin (BEA), and enniatins (ENNs). Fusarium proliferatum strains produced FBs at high amount, and, at a lesser extent, BEA, and ENNs; F.sacchari produced only BEA and B ENNs at very low level; Fusarium sp. strains produced only B ENNs. The paper provides new insights on the genetic diversity of Fusarium species and their mycotoxin profile occurring on sugarcane in Iran.
甘蔗是伊朗南部农业食品、能源和制药工业的重要作物。在侵染甘蔗的病原体中，产毒素镰刀菌是一个严重的问题，因为它们对植物具有致病性，并且能够产生对人类和动物有害的真菌毒素。我们研究了 104 株从伊朗南部甘蔗中分离出的镰刀菌菌株，进行了分子鉴定、系统发育和真菌毒素分析。大多数镰刀菌菌株属于尖孢镰刀菌复合种（FFSC），主要鉴定为层出镰刀菌，少量为串珠镰刀菌，很少为唐菖蒲镰刀菌和轮枝镰刀菌。此外，14 株鉴定为 FFSC 的菌株虽然与 F. andiyazi 在系统发育上密切相关，但无法归为任何已知种，可能代表一个新的系统发育种。对一部分 FFSC 菌株进行了体外产伏马菌素（FBs）、 beauvericin（BEA）和 enniatins（ENNs）的分析。层出镰刀菌菌株大量产生 FBs，少量产生 BEA 和 ENNs；串珠镰刀菌仅产生 BEA 和 B ENNs，且含量非常低； Fusarium sp. 菌株仅产生 B ENNs。本文为伊朗甘蔗上镰刀菌的遗传多样性及其真菌毒素特征提供了新的见解。
