Department of Pharmaceutical Sciences, Faculty of Science and Engineering, Dibrugarh University, Dibrugarh, Assam 786004, India.
Wellcome Trust- DBT (India Alliance) Lab, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.
CNS Neurol Disord Drug Targets. 2022;21(4):335-342. doi: 10.2174/1871527320666210827114227.
Dementia correlates with Alzheimer's disease, Parkinson's disease, frontotemporal and cerebrovascular diseases. There are supporting shreds of evidence on the pharmacological activity of (Zingiberaceae family) for its antioxidant, antidepressant, analgesic, anticonvulsant, and anti-acetylcholinesterase effect.
This study aims to analyze the fecal microbial profile in Zederone treated demented rat model.
In our study, isolation and characterization of Zederone were carried out from rhizomes, followed by estimation of its memory-enhancing effect on Aluminium-induced demented rat, which was evaluated by behavioural study on radial 8 arm maze. Moreover the detection of amyloid plaque formation was carried out using fluorescent microscopy of the congo red-stained rat brain tissues of the cerebral neocortex region. This study included eighteen female Wistar Albino rats that were divided into three groups that consisted of six rats in each group. The study of fecal microbial profile by metagenomic DNA extraction followed by next-generation sequencing was carried out to establish the correlation between gut microbes and amyloid plaques in dementia.
Zederone could be characterized as pale yellow colored, needle-shaped crystals with 96.57% purity. This compound at 10 mg/kg body weight showed cognition improving capacity (p ≤ 0.0001) with a reduction of accumulated amyloid plaques that were detected in the demented group in fluorescence microscope and fecal microbiome study divulged an increased shift towards genera in the treated group from in the demented group.
This sesquiterpenoid compound would assist in the modulation of gut bacterial dysbiosis and act as a better therapeutic drug for dementia and other neurological disorders.
痴呆与阿尔茨海默病、帕金森病、额颞叶和脑血管疾病有关。有证据表明，（姜科）具有抗氧化、抗抑郁、镇痛、抗惊厥和抗乙酰胆碱酯酶作用。
本研究旨在分析 Zederone 治疗痴呆大鼠模型的粪便微生物特征。
在我们的研究中，从根茎中分离和鉴定 Zederone，并评估其对铝诱导痴呆大鼠的记忆增强作用，这通过在放射状 8 臂迷宫上的行为研究来评估。此外，通过刚果红染色的大鼠大脑皮质区域的荧光显微镜检测淀粉样斑块的形成。本研究包括 18 只雌性 Wistar 白化大鼠，分为三组，每组 6 只。通过宏基因组 DNA 提取和下一代测序进行粪便微生物特征研究，以建立肠道微生物与痴呆症中淀粉样斑块之间的相关性。
Zederone 可被鉴定为浅黄色、针状晶体，纯度为 96.57%。该化合物在 10mg/kg 体重时表现出认知改善能力（p≤0.0001），并减少了在荧光显微镜下检测到的痴呆组中的累积淀粉样斑块，粪便微生物组研究表明，治疗组中 属的比例增加，而痴呆组中为 属。
这种倍半萜化合物将有助于调节肠道细菌失调，并作为治疗痴呆症和其他神经退行性疾病的更好治疗药物。
