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环状RNA circPTK2可能通过抑制miR-23a成熟来抑制胶质母细胞瘤癌细胞的侵袭和迁移。

CircRNA circPTK2 Might Suppress Cancer Cell Invasion and Migration of Glioblastoma by Inhibiting miR-23a Maturation.

作者信息

Chen Wei, Wang Ning, Lian Minxue

机构信息

Department of Neurosurgery, First Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, Shanxi Province, 710061, People's Republic of China.

出版信息

Neuropsychiatr Dis Treat. 2021 Aug 21;17:2767-2774. doi: 10.2147/NDT.S297108. eCollection 2021.

DOI:10.2147/NDT.S297108
PMID:34456566
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8387247/
Abstract

BACKGROUND

CircRNA circPTK2 plays opposite roles in different cancers, while its role in glioblastoma is unknown. The aim of this study was to explore the involvement of circPTK2 in glioblastoma.

METHODS

Expression of circPTK2, mature miR-23a, and premature miR-23a in paired cancer and non-cancer tissues from glioblastoma patients (n = 60) was analyzed by RT-qPCR. Pearson's correlation coefficient was used to analyze the correlations between gene expressions. The effects of circPTK2 overexpression on miR-23a maturation were analyzed by transfecting circPTK2 expression vector into glioblastoma cells, followed by determining the expression of mature miR-23a and premature miR-23a by RT-qPCR. Transwell assays were carried out to explore the role of circPTK2 and miR-23a in regulating glioblastoma cell invasion and migration.

RESULTS

We found that circPTK2 was downregulated in GBM and was inversely correlated with mature miR-23a, but not premature miR-23a. GBM cells transfected with circPTK2 expression vector showed significantly downregulated mature miR-23a, but not premature miR-23a. Transwell assay analysis showed that circPTK2 overexpression decreased cell invasion and migration, while miR-23a increased cell invasion and migration. Moreover, miR-23a overexpression reversed the inhibitory effects of circPTK2 overexpression on cell behaviors.

CONCLUSION

CircPTK2 might suppress cancer cell invasion and migration by inhibiting the maturation of miR-23a.

摘要

背景

环状RNA circPTK2在不同癌症中发挥相反作用，但其在胶质母细胞瘤中的作用尚不清楚。本研究旨在探讨circPTK2在胶质母细胞瘤中的作用。

方法

采用RT-qPCR分析胶质母细胞瘤患者（n = 60）配对的癌组织和非癌组织中circPTK2、成熟miR-23a和前体miR-23a的表达。用Pearson相关系数分析基因表达之间的相关性。将circPTK2表达载体转染到胶质母细胞瘤细胞中，然后通过RT-qPCR测定成熟miR-23a和前体miR-23a的表达，分析circPTK2过表达对miR-23a成熟的影响。进行Transwell实验，探讨circPTK2和miR-23a在调节胶质母细胞瘤细胞侵袭和迁移中的作用。

结果

我们发现circPTK2在胶质母细胞瘤中表达下调，且与成熟miR-23a呈负相关，但与前体miR-23a无关。用circPTK2表达载体转染的胶质母细胞瘤细胞显示成熟miR-23a显著下调，但前体miR-23a无明显变化。Transwell实验分析表明，circPTK2过表达降低细胞侵袭和迁移，而miR-23a增加细胞侵袭和迁移。此外，miR-23a过表达逆转了circPTK2过表达对细胞行为的抑制作用。

结论

CircPTK2可能通过抑制miR-23a的成熟来抑制癌细胞的侵袭和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/400795664585/NDT-17-2767-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/881a9b6592a9/NDT-17-2767-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/d99b89c66777/NDT-17-2767-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/acefafc4e9e0/NDT-17-2767-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/400795664585/NDT-17-2767-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/881a9b6592a9/NDT-17-2767-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/d99b89c66777/NDT-17-2767-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/acefafc4e9e0/NDT-17-2767-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec60/8387247/400795664585/NDT-17-2767-g0004.jpg

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