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环状RNA circNF1的siRNA沉默通过促进miR-340的成熟来抑制胶质母细胞瘤细胞增殖。

Circular RNA circNF1 siRNA Silencing Inhibits Glioblastoma Cell Proliferation by Promoting the Maturation of miR-340.

作者信息

Liu Li, Jia Li, Shao Jun, Liu Hanhua, Wu Qinke, Wu Xudong

机构信息

Taizhou First People's Hospital, Taizhou, China.

Department of Urology, Yidu Central Hospital of Weifang City, Weifang, China.

出版信息

Front Neurol. 2021 Sep 13;12:658076. doi: 10.3389/fneur.2021.658076. eCollection 2021.

DOI:10.3389/fneur.2021.658076
PMID:34589042
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8475906/
Abstract

It has been reported that circNF1, a type of circular RNA (circRNA), promotes gastric cancer. This study aimed to analyze the role of circNF1 in glioblastoma (GBM). The expression of circNF1, mature miR-340, and miR-340 precursor in paired GBM and non-cancer tissues from GBM patients ( = 50) was analyzed by RT-qPCR. GBM cells were transfected with circNF1 siRNA, followed by the analysis of the expression of mature miR-340 and miR-340 precursor, to study the effects of circNF1 knockdown on the maturation of miR-340. The CCK-8 assay was carried out to explore the role of circNF1 and miR-340 in the proliferation of GBM cells. circNF1 expression was found to be upregulated in GBM and was correlated with patient survival. In glioma tissue, circNF1 was inversely correlated with mature miR-340, but not with the miR-340 precursor. In GBM cells, circNF1 siRNA silencing resulted in the upregulation of mature miR-340, but not the miR-340 precursor. The cell proliferation assay showed that circNF1 siRNA silencing and miR-340 overexpression decreased the proliferation of GBM cells. In addition, the miR-340a inhibitor suppressed the role of circNF1 siRNA silencing in cell proliferation. Therefore, circNF1 siRNA silencing may inhibit GBM cell proliferation by promoting the maturation of miR-340.

摘要

据报道，环状RNA（circRNA）中的circNF1可促进胃癌。本研究旨在分析circNF1在胶质母细胞瘤（GBM）中的作用。通过RT-qPCR分析了50例GBM患者配对的GBM组织和非癌组织中circNF1、成熟miR-340和miR-340前体的表达。用circNF1 siRNA转染GBM细胞，随后分析成熟miR-340和miR-340前体的表达，以研究circNF1敲低对miR-340成熟的影响。进行CCK-8实验以探究circNF1和miR-340在GBM细胞增殖中的作用。发现circNF1在GBM中表达上调，且与患者生存率相关。在胶质瘤组织中，circNF1与成熟miR-340呈负相关，但与miR-340前体无关。在GBM细胞中，circNF1 siRNA沉默导致成熟miR-340上调，但miR-340前体未上调。细胞增殖实验表明，circNF1 siRNA沉默和miR-340过表达降低了GBM细胞的增殖。此外，miR-340a抑制剂抑制了circNF1 siRNA沉默在细胞增殖中的作用。因此，circNF1 siRNA沉默可能通过促进miR-340的成熟来抑制GBM细胞增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/25ce634ad9c6/fneur-12-658076-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/ca8609882fec/fneur-12-658076-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/4486a990cdd3/fneur-12-658076-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/ce60326d4d75/fneur-12-658076-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/20a90d083951/fneur-12-658076-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/d26b125bf29d/fneur-12-658076-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/25ce634ad9c6/fneur-12-658076-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/ca8609882fec/fneur-12-658076-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/4486a990cdd3/fneur-12-658076-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/ce60326d4d75/fneur-12-658076-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/20a90d083951/fneur-12-658076-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/d26b125bf29d/fneur-12-658076-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ae9/8475906/25ce634ad9c6/fneur-12-658076-g0006.jpg

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