Zhang Xuemei, Zong Yuanyuan, Gong Di, Yu Lirong, Sionov Edward, Bi Yang, Prusky Dov
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel.
Front Plant Sci. 2021 Aug 12;12:696210. doi: 10.3389/fpls.2021.696210. eCollection 2021.
The occurrence of reactive oxygen species (ROS) during the colonization of necrotrophic pathogens attacking fruit is critical during the attack, but its importance in remains unclear. This study aimed to determine the regulatory effects of NADPH oxidase (Nox) genes on the growth and pathogenicity of in apple fruits. Deletion mutants of Δ, Δ, and Δ genes were constructed to determine the contribution to the colonization process. The Δ strain had a significant effect on the reduction of growth and pathogenicity, the Δ strain negatively regulated the growth and development of and did not show any significant effect on the pathogenicity, and the Δ strain showed no effect on the growth or pathogenicity of in the apple fruits. However, analysis of the content of O and HO in the mycelium of all the Nox mutants showed a significant reduction, confirming the functionality of Nox mutations. Growth under stress conditions in the presence of Congo red, sodium lauryl sulfate, and HO showed a negative effect on the radial growth of Δ, but a positive effect on radial growth reduction by Δ and Δ mutants was shown. Interestingly, the host antioxidant activity levels of superoxide dismutase (SOD) andcatalase (CAT) in the fruits after inoculation with Δ, Δ, and Δ mutants declined, suggesting reduced ROS accumulation in the colonized region. These results suggest that , , and differentially regulate the growth and pathogenicity of by producing ROS, and that showed the strongest regulatory effect.
在侵袭果实的坏死营养型病原菌定殖过程中，活性氧物质（ROS）的产生在侵染过程中至关重要，但其在[具体方面]的重要性仍不清楚。本研究旨在确定NADPH氧化酶（Nox）基因对苹果果实中[病原菌名称]生长和致病性的调控作用。构建了Δ[基因名称1]、Δ[基因名称2]和Δ[基因名称3]基因的缺失突变体，以确定其对定殖过程的贡献。Δ[基因名称1]菌株对生长和致病性的降低有显著影响，Δ[基因名称2]菌株对[病原菌名称]的生长发育有负调控作用，且对致病性无显著影响，Δ[基因名称3]菌株对苹果果实中[病原菌名称]的生长或致病性无影响。然而，对所有Nox突变体菌丝体中O₂⁻和H₂O₂含量的分析表明显著降低，证实了Nox突变的功能性。在刚果红、十二烷基硫酸钠和H₂O₂存在的应激条件下生长，对Δ[基因名称1]的径向生长有负面影响，但对Δ[基因名称2]和Δ[基因名称3]突变体的径向生长减少有正面影响。有趣的是，接种Δ[基因名称1]、Δ[基因名称2]和Δ[基因名称3]突变体后，果实中宿主超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的抗氧化活性水平下降，表明定殖区域中ROS积累减少。这些结果表明，[基因名称1]、[基因名称2]和[基因名称3]通过产生活性氧物质差异调节[病原菌名称]的生长和致病性，且[基因名称1]显示出最强的调控作用。
