He Xiaoyan, Han Zhen, Yin Huayan, Chen Fan, Dong Yihuan, Zhang Lufei, Lu Xiaoqing, Zeng Jianbin, Ma Wujun, Mu Ping
College of Agronomy, Qingdao Agricultural University, Qingdao, China.
State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
Front Genet. 2021 Aug 11;12:724527. doi: 10.3389/fgene.2021.724527. eCollection 2021.
Soil salinization is one of the major abiotic stresses that adversely affect the yield and quality of crops such as wheat, a leading cereal crop worldwide. Excavating the salt-tolerant genes and exploring the salt tolerance mechanism can help breeding salt-tolerant wheat varieties. Thus, it is essential to identify salt-tolerant wheat germplasm resources. In this study, we carried out a salt stress experiment using Qing Mai 6 (QM6), a salt-tolerant wheat variety, and sequenced the miRNAs and mRNAs. The differentially expressed miRNAs and mRNAs in salt stress conditions were compared with the control. As results, a total of eight salt-tolerance-related miRNAs and their corresponding 11 target mRNAs were identified. Further analysis revealed that QM6 enhances salt tolerance through increasing the expression level of genes related to stress resistance, antioxidation, nutrient absorption, and lipid metabolism balance, and the expression of these genes was regulated by the identified miRNAs. The resulting data provides a theoretical basis for future research studies on miRNAs and novel genes related to salt tolerance in wheat in order to develop genetically improved salt-tolerant wheat varieties.
土壤盐渍化是主要的非生物胁迫之一，对全球主要谷类作物小麦等农作物的产量和品质产生不利影响。挖掘耐盐基因并探索耐盐机制有助于培育耐盐小麦品种。因此，鉴定耐盐小麦种质资源至关重要。在本研究中，我们使用耐盐小麦品种青麦6（QM6）进行了盐胁迫实验，并对miRNA和mRNA进行了测序。将盐胁迫条件下差异表达的miRNA和mRNA与对照进行比较。结果，共鉴定出8个与耐盐相关的miRNA及其相应的11个靶mRNA。进一步分析表明，QM6通过提高与抗逆、抗氧化、养分吸收和脂质代谢平衡相关基因的表达水平来增强耐盐性，这些基因的表达受所鉴定的miRNA调控。所得数据为未来关于小麦中与耐盐性相关的miRNA和新基因的研究提供了理论基础，以便培育基因改良的耐盐小麦品种。
