Qiburi Qiburi, Temuqile Temuqile, Baigude Huricha
Institute of Mongolian Medicinal Chemistry, School of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot 010020, Inner Mongolia, China.
International Hospital of Mongolian Medicine, Hohhot 010021, Inner Mongolia, China.
Evid Based Complement Alternat Med. 2021 Aug 18;2021:9920364. doi: 10.1155/2021/9920364. eCollection 2021.
The activated microglia contribute to stroke-induced neuroinflammation by upregulating the expression of a pleura of genes that are characterized as either proinflammatory or anti-inflammatory. The natural products alantolactone (Ala) and dehydrodiisoeugenol (Deh) found in L. and Houtt., respectively, are regularly used in traditional herb medicine, which play anti-inflammatory and antioxidant roles via regulation of canonical pathways such as nuclear factor kappa B (NF-B) in microglia and microphages. To illustrate the full spectra of gene expression alteration in microglia treated with Ala, Deh, and the mixture of Ala and Deh (denoted as Mix), we performed RNA-seq analysis of total RNA extracted from lipopolysaccharide- (LPS-) treated microglia subsequently exposed to Ala, Deh, and Mix. While both chemicals regulated the gene expression that facilitates an anti-inflammatory polarization, the mixture exerted some distinctive synergic regulatory effect, which differed from either of the chemicals alone. Our data provide important evidence for further research on the therapeutic mechanism of traditional medicine including Eerdun Wurile (EW).
活化的小胶质细胞通过上调一系列具有促炎或抗炎特征的基因的表达，促进中风诱导的神经炎症。分别在土木香和细辛中发现的天然产物土木香内酯（Ala）和去氢二异丁香酚（Deh）常用于传统草药，它们通过调节小胶质细胞和巨噬细胞中的核因子κB（NF-κB）等经典途径发挥抗炎和抗氧化作用。为了阐明用Ala、Deh以及Ala和Deh的混合物（称为Mix）处理的小胶质细胞中基因表达变化的全貌，我们对从脂多糖（LPS）处理的小胶质细胞中提取的总RNA进行了RNA测序分析，并随后将其分别暴露于Ala、Deh和Mix。虽然这两种化学物质都调节了促进抗炎极化的基因表达，但混合物发挥了一些独特的协同调节作用，这与单独使用任何一种化学物质都不同。我们的数据为包括额尔敦乌日勒（EW）在内的传统医学治疗机制的进一步研究提供了重要证据。
