Li Yang, Hu Ping, Wang Xiali, Hou Xu, Liu Fengzhen, Jiang Xiaohong
Zhong Yuan Academy of Biological Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng 252000, China.
Clinical Laboratory, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng 252000, China.
Regen Biomater. 2021 Aug 10;8(5):rbab046. doi: 10.1093/rb/rbab046. eCollection 2021 Oct.
Photothermal therapy (PTT) has emerged as a promising cancer therapeutic method. In this study, Arg-Gly-Asp (RGD) peptide-conjugated polydopamine-coated gold nanostars (Au@PDA-RGD NPs) were prepared for targeting PTT of hepatocellular carcinoma (HCC). A polydopamine (PDA) shell was coated on the surface of gold nanostars by the oxidative self-polymerization of dopamine (termed as Au@PDA NPs). Au@PDA NPs were further functionalized with polyethylene glycol and RGD peptide to improve biocompatibility as well as selectivity toward the HCC cells. Au@PDA-RGD NPs showed an intense absorption at 822 nm, which makes them suitable for near-infrared-excited PTT. Our results indicated that the Au@PDA-RGD NPs were effective for the PTT therapy of the αβ integrin receptor-overexpressed HepG2 cells . Further antitumor mechanism studies showed that the Au@PDA-RGD NPs-based PTT induced human liver cancer cells death via the mitochondrial-lysosomal and autophagy pathways. experiments showed that Au@PDA-RGD NPs had excellent tumor treatment efficiency and negligible side effects. Thus, our study showed that Au@PDA-RGD NPs could offer an excellent nanoplatform for PTT of HCC.
光热疗法（PTT）已成为一种很有前景的癌症治疗方法。在本研究中，制备了精氨酸-甘氨酸-天冬氨酸（RGD）肽偶联的聚多巴胺包覆金纳米星（Au@PDA-RGD NPs）用于肝细胞癌（HCC）的靶向光热治疗。通过多巴胺的氧化自聚合在金纳米星表面包覆一层聚多巴胺（PDA）壳（称为Au@PDA NPs）。Au@PDA NPs进一步用聚乙二醇和RGD肽功能化，以提高生物相容性以及对肝癌细胞的选择性。Au@PDA-RGD NPs在822nm处有强烈吸收，这使其适用于近红外激发的光热治疗。我们的结果表明，Au@PDA-RGD NPs对αβ整合素受体过表达的HepG2细胞的光热治疗有效。进一步的抗肿瘤机制研究表明，基于Au@PDA-RGD NPs的光热治疗通过线粒体-溶酶体和自噬途径诱导人肝癌细胞死亡。实验表明，Au@PDA-RGD NPs具有优异的肿瘤治疗效果且副作用可忽略不计。因此，我们的研究表明，Au@PDA-RGD NPs可为肝癌的光热治疗提供一个优异的纳米平台。
